Detailed information of TA system TA167516

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4724103..4724705
• Replicon: chromosome
• Accession: NZ_CP058790
• Organism: Shigella flexneri strain STLIN_6

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: HZT08_RS22870
• Protein ID: WP_000897305.1
• Coordinates: 4724394..4724705 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HZT08_RS22865
• Protein ID: WP_000356397.1
• Coordinates: 4724103..4724393 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZT08_RS22840 (4720030)	4720030..4720932	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
HZT08_RS22845 (4720929)	4720929..4721564	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
HZT08_RS22850 (4721561)	4721561..4722490	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
HZT08_RS22855 (4722820)	4722820..4723062	-	243	WP_001087409.1	protein YiiF	-
HZT08_RS22860 (4723280)	4723280..4723498	-	219	Protein_4469	ribbon-helix-helix domain-containing protein	-
HZT08_RS22865 (4724103)	4724103..4724393	-	291	WP_000356397.1	NadS family protein	Antitoxin
HZT08_RS22870 (4724394)	4724394..4724705	-	312	WP_000897305.1	hypothetical protein	Toxin
HZT08_RS22875 (4724934)	4724934..4725842	+	909	WP_001299484.1	alpha/beta hydrolase	-
HZT08_RS22880 (4725906)	4725906..4726847	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
HZT08_RS22885 (4726892)	4726892..4727329	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
HZT08_RS22890 (4727326)	4727326..4728198	-	873	WP_000920762.1	virulence factor BrkB family protein	-
HZT08_RS22895 (4728192)	4728192..4728791	-	600	WP_001295269.1	glucose-1-phosphatase	-
HZT08_RS22900 (4728890)	4728890..4729675	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T167516 WP_000897305.1 NZ_CP058790:c4724705-4724394 [Shigella flexneri]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T167516 NZ_CP075140:471195-471491 [Salmonella enterica]
ATGATTGACATAAAAACAACACCAGAGGCCGAGAAGTGGCTAAAGGGATTGAAAGACCGGGTAGCGAAATCAAAGATATT
AATCAGAATCGACAGAATGAGAACGGGGAACTTTGGTGATGTTGAGCCAGTAGGCGGCGGGTTATCTGAATTGCGCATAC
ATCACGGCAAAGGTTATCGGGTTTATTTTGGGAATAAAAACAACGAAATAATATTAATTCTTTGTGGCGGGGATAAAAGC
ACACAGCAGGATGATATACAGAAAGCGAAACAGATAGCCAAAAAATGGGGGTTTTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT167516 WP_000356397.1 NZ_CP058790:c4724393-4724103 [Shigella flexneri]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT167516 NZ_CP075140:471493-471768 [Salmonella enterica]
ATGAAACAGGTATTAAAAGATTTTGACGTCGCAGACTATTTAGAGGACGAGGATGATGTTATCGCTTATCTAAATGCCGT
TATTGCCGAGGGTGACAATAAACAACTTTTCATTGCGCTGGGCAACATTGCAAGAGCAAGAAATATAAGCCGTATTTCAA
AAGAAACCGGAATCAGCAGGGAGGGAATTTATAAAGCACTTTCAGGCGATGGAAATCCATCATTTAACACCCTTTCTAAA
ATAACTAAAGCGATGGGGTTAGATCTTCACTTTTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7