Detailed information of TA system TA167489

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 49329..50040
• Replicon: plasmid unnamed1
• Accession: NZ_CP058787
• Organism: Shigella flexneri strain STLIN_4

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I2UJZ0
• Locus tag: HZT07_RS23240
• Protein ID: WP_000162415.1
• Coordinates: 49329..49631 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HZT07_RS23245
• Protein ID: WP_000806445.1
• Coordinates: 49702..50040 (+)
• Length: 113 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZT07_RS23210 (HZT07_23050)	44348..44998	-	651	WP_240763466.1	hypothetical protein	-
HZT07_RS23215 (HZT07_23055)	45012..45917	-	906	WP_001258014.1	recombination-associated protein RdgC	-
HZT07_RS23220 (HZT07_23060)	45977..46630	-	654	WP_000828873.1	hypothetical protein	-
HZT07_RS23225 (HZT07_23065)	46627..47607	-	981	WP_000046499.1	hypothetical protein	-
HZT07_RS23230 (HZT07_23070)	47611..48378	-	768	WP_000203290.1	hypothetical protein	-
HZT07_RS23235 (HZT07_23075)	48375..49166	-	792	WP_000532305.1	hypothetical protein	-
HZT07_RS23240 (HZT07_23080)	49329..49631	+	303	WP_000162415.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HZT07_RS23245 (HZT07_23085)	49702..50040	+	339	WP_000806445.1	HigA family addiction module antitoxin	Antitoxin
HZT07_RS23250 (HZT07_23090)	50097..50282	+	186	Protein_56	hypothetical protein	-
HZT07_RS23255 (HZT07_23095)	50319..51047	+	729	WP_000986754.1	hypothetical protein	-
HZT07_RS23260 (HZT07_23100)	51081..52274	-	1194	WP_240763458.1	phage tail protein	-
HZT07_RS23265 (HZT07_23105)	52267..52596	-	330	WP_000542383.1	hypothetical protein	-
HZT07_RS23270 (HZT07_23110)	52925..53578	-	654	WP_077882283.1	maturation control protein	-
HZT07_RS23275 (HZT07_23115)	53862..54470	+	609	WP_000776951.1	hypothetical protein	-
HZT07_RS23280	54491..54883	+	393	WP_000824759.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..92052	92052

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (6-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-69)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11818.56 Da
• Isoelectric Point: 9.8739

>T167489 WP_000162415.1 NZ_CP058787:49329-49631 [Shigella flexneri]
MTKKINIKDFRDAWLDDFFEFSTPHRKIPPDIHMTLSRKLDIINAATTCKDLRSPPGNRYEELSGKLNGYSSVRVNKQYR
LIFKWVNGKAEDLYLDPHKY

Nucleotide

• Length: 303 bp

>T167489 NZ_CP075136:c10891-10577 [Salmonella enterica]
ATGCAGTTCACTGTATACAGTAACACCGGAAAGAGCACCGTCTATCCCCTGCTGCTTGATGTAACCAGCGATATTATCGG
CCAGTTGAACCGCAGGATTGTGATCCCGCTGCTGCCTGTTGAAAAGTACCCAGGCAGCACCCGCCCGGAACGGCTGGTTC
CGCTTGTCAGACTGACCGACGATAAAGAATATGCAGTTATGACGCATGAAATGGCGAGTATTCCCGCCCGTTCTCTGGGG
GCTGCGTTCTGCGATGCTTCCCCTTATCGTACCGAAGTCAAAGCTGCGATTGATTTTCTTCTTGATGGTATTTAA

Antitoxin

Protein

• Length: 113 a.a.
• Molecular weight: 13049.98 Da
• Isoelectric Point: 6.4754

>AT167489 WP_000806445.1 NZ_CP058787:49702-50040 [Shigella flexneri]
MKQATRKPTTVGDILLYEYLEPLELKINELAEILHVHRNTVSALVNNNRKLTMDMAYRLAKAFDTSVDFWINLQTAVDLW
EVENDMRVQEELSRINTAEKFISQRNLNKKAA

Nucleotide

• Length: 339 bp

>AT167489 NZ_CP075136:c11130-10894 [Salmonella enterica]
ATGGCAACAACACAACGAGCAACGCAAAGCGTTACTATGACTGTAGAACGTGCCTTACTCAGCCGTGCGCGTGAGGCTGG
AATTAATCTCAGTGCCACTCTGACCGCAGCCCTTGACGCTGAACTACGCCAGTATGAAGCGAAAAAATGGCAGGAGGAAA
ACAACGAAGCGCTGGAAGCTCTTAATCGTTTTCACGATGAACATGGTTGTTTCAGCGATGAGTACAGGACATTTTAA

Structures

Toxin

• AlphaFold DB: A0A631VBQ3