Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3686615..3687309 Replicon chromosome
Accession NZ_CP058786
Organism Shigella flexneri strain STLIN_4

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag HZT07_RS18085 Protein ID WP_001263489.1
Coordinates 3686615..3687013 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag HZT07_RS18090 Protein ID WP_000554758.1
Coordinates 3687016..3687309 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (3682203) 3682203..3682283 - 81 NuclAT_11 - -
- (3682203) 3682203..3682283 - 81 NuclAT_11 - -
- (3682203) 3682203..3682283 - 81 NuclAT_11 - -
- (3682203) 3682203..3682283 - 81 NuclAT_11 - -
HZT07_RS18060 (3682879) 3682879..3683337 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
HZT07_RS18065 (3683598) 3683598..3685055 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
HZT07_RS18070 (3685112) 3685112..3685633 - 522 Protein_3549 peptide chain release factor H -
HZT07_RS18075 (3685629) 3685629..3685835 - 207 Protein_3550 RtcB family protein -
HZT07_RS18080 (3686153) 3686153..3686605 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
HZT07_RS18085 (3686615) 3686615..3687013 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
HZT07_RS18090 (3687016) 3687016..3687309 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
HZT07_RS18095 (3687361) 3687361..3688416 - 1056 WP_001226164.1 DNA polymerase IV -
HZT07_RS18100 (3688487) 3688487..3689272 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
HZT07_RS18105 (3689244) 3689244..3690956 + 1713 Protein_3556 flagellar biosynthesis protein FlhA -
HZT07_RS18110 (3691180) 3691180..3691677 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T167485 WP_001263489.1 NZ_CP058786:c3687013-3686615 [Shigella flexneri]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T167485 NZ_CP075135:c4246623-4246339 [Salmonella enterica]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATTGACTCTGCCCGTTTAAACGGTCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGTCTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTCGCGGTCGGCAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT167485 WP_000554758.1 NZ_CP058786:c3687309-3687016 [Shigella flexneri]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT167485 NZ_CP075135:c4246855-4246613 [Salmonella enterica]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAGGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAGGATGCACTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References