Detailed information of TA system TA167445

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 731346..732039
• Replicon: chromosome
• Accession: NZ_CP058780
• Organism: Shigella flexneri strain STLIN_17

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: HZT03_RS03605
• Protein ID: WP_000415584.1
• Coordinates: 731346..731642 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: HZT03_RS03610
• Protein ID: WP_000650107.1
• Coordinates: 731644..732039 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZT03_RS03570 (726434)	726434..726748	-	315	WP_000958598.1	putative quinol monooxygenase	-
HZT03_RS03575 (726779)	726779..727360	-	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
HZT03_RS03580 (727679)	727679..728011	+	333	WP_000917684.1	DUF2645 family protein	-
HZT03_RS03585 (728057)	728057..729406	-	1350	WP_000673402.1	quorum sensing histidine kinase QseC	-
HZT03_RS03590 (729403)	729403..730062	-	660	WP_001221493.1	quorum sensing response regulator transcription factor QseB	-
HZT03_RS03595 (730214)	730214..730606	+	393	WP_240764237.1	OB fold stress tolerance protein YgiW	-
HZT03_RS03600 (730659)	730659..731141	+	483	WP_000183505.1	GyrI-like domain-containing protein	-
HZT03_RS03605 (731346)	731346..731642	+	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
HZT03_RS03610 (731644)	731644..732039	+	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
HZT03_RS03615 (732172)	732172..733779	+	1608	WP_001295629.1	ABC transporter substrate-binding protein	-
HZT03_RS03620 (733917)	733917..736175	+	2259	WP_001281881.1	DNA topoisomerase IV subunit A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T167445 WP_000415584.1 NZ_CP058780:731346-731642 [Shigella flexneri]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T167445 NZ_CP075129:938366-938779 [Salmonella enterica subsp. enterica serovar Rubislaw]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGCCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCGTCCGCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CGCGCCAACACTGA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT167445 WP_000650107.1 NZ_CP058780:731644-732039 [Shigella flexneri]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT167445 NZ_CP075129:938119-938385 [Salmonella enterica subsp. enterica serovar Rubislaw]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGATGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• PDB: 3HI2

Antitoxin

• PDB: 3HI2
• PDB: 3GA8
• PDB: 3O9X
• PDB: 2KZ8
• PDB: 3FMY
• PDB: 3GN5
• AlphaFold DB: A0A7U9LU65