Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 611517..612244 Replicon chromosome
Accession NZ_CP058780
Organism Shigella flexneri strain STLIN_17

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag HZT03_RS03000 Protein ID WP_000550189.1
Coordinates 611517..611831 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag HZT03_RS03005 Protein ID WP_000560255.1
Coordinates 611828..612244 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT03_RS02980 (607683) 607683..608669 - 987 WP_094337096.1 Gfo/Idh/MocA family oxidoreductase -
HZT03_RS02985 (608748) 608748..609431 - 684 WP_001183041.1 vancomycin high temperature exclusion protein -
HZT03_RS02990 (609508) 609508..610011 - 504 WP_001295542.1 M48 family metallopeptidase -
HZT03_RS02995 (610096) 610096..611232 + 1137 WP_000018690.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
HZT03_RS03000 (611517) 611517..611831 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
HZT03_RS03005 (611828) 611828..612244 + 417 WP_000560255.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
HZT03_RS03010 (612289) 612289..614307 - 2019 WP_094337095.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
HZT03_RS03015 (614633) 614633..616984 - 2352 WP_219359307.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Antitoxin HigA

(1-138)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T167443 WP_000550189.1 NZ_CP058780:611517-611831 [Shigella flexneri]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T167443 NZ_CP075128:4995318-4995629 [Salmonella enterica subsp. diarizonae serovar 60:r:e,n,x,z15]
GTGCATGTTATCAGCCGAAAACCGTTCAATGAAGCGATGCTCATGTATCCAAACCATGAGCTTGCCCTCACTGAGTTGCT
GAATGTTCTTGAGAAAAAAACATTCACTCAGCCTGAAGAGATGAAGCGGTATATTCCTTCACTGGATAATTTTAAGTACA
GAGATAAATGGTGGGTGATTGATGTTTCTGGTAATAGCCTGAGACTCATTTCCTACATAGATTTCAGGTTACATAAGATA
TTTGTGAAACATATCGTTTCCCATGCCGAATATGACAAACTGACCGCATATTATCGGGGTAATAAAGAATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14951.41 Da        Isoelectric Point: 4.5577

>AT167443 WP_000560255.1 NZ_CP058780:611828-612244 [Shigella flexneri]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNAPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT167443 NZ_CP075128:4995626-4996072 [Salmonella enterica subsp. diarizonae serovar 60:r:e,n,x,z15]
ATGAGAACTCATCGTCAGATGGATGCAACCAGCGCAAAAAAGATCGTTGATACCTTCAGCGATGCGGTAAAAACCGTCCC
ACTGATGGGGGAAGACCGAAATGACAATGAGTATCGCAGGGCACTAGCGCTAGTGGAGTTTCTGGTCGACCACGACGATC
TTGAAAACCCACTATTTGAATTGCTCTGTGCCCGAATCAGTGAATACGAAAAACATGCGCCGGAATTCAAAGCACTCAAC
CAACACCTGGAGAAAACGCCCCCTGGCGTTTCAGTATTGCGAACGCTCATGGATCAATACGGTCTCAAAGCAGCAGATCT
TGCCAACGAACTTGGTTCTAAATCGAACGTCAGCAACATCTTAAATGGCCGCAGAGCACTAACGGTTAATCATATTAAAG
CGCTTACACAACGCTTTAAACTACCAGCGGATGCCTTCATCGAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References