Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 562004..562803 Replicon chromosome
Accession NZ_CP058780
Organism Shigella flexneri strain STLIN_17

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag HZT03_RS02745 Protein ID WP_000347251.1
Coordinates 562004..562468 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag HZT03_RS02750 Protein ID WP_001307405.1
Coordinates 562468..562803 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT03_RS02720 (557521) 557521..558381 + 861 WP_000809256.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
HZT03_RS02725 (558424) 558424..559515 - 1092 WP_094337107.1 fimbrial protein -
HZT03_RS02730 (559526) 559526..561067 - 1542 Protein_535 fimbrial biogenesis outer membrane usher protein -
HZT03_RS02735 (561124) 561124..561821 + 698 WP_103215986.1 IS1-like element IS1A family transposase -
HZT03_RS02740 (561829) 561829..561949 + 121 Protein_537 DeoR family transcriptional regulator -
HZT03_RS02745 (562004) 562004..562468 - 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
HZT03_RS02750 (562468) 562468..562803 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
HZT03_RS02755 (562952) 562952..564523 - 1572 WP_001273753.1 galactarate dehydratase -
HZT03_RS02760 (564898) 564898..566232 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
HZT03_RS02765 (566248) 566248..567018 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 561444..573573 12129


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T167442 WP_000347251.1 NZ_CP058780:c562468-562004 [Shigella flexneri]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T167442 NZ_CP075128:c4993859-4993485 [Salmonella enterica subsp. diarizonae serovar 60:r:e,n,x,z15]
ATGGTCAAAGGGTCTGCGCTTTTTGATACCAATATCCTCATCGACCTGTTTAGCGGTCGTATTGAAGCGAAACATGCGCT
GGAAGCGTATCCGCCACAGAATGCGATTAGCCTTATCACATGGATGGAAGTGATGGTGGGCGCTAAGAAATATCATCAGG
AAAACCGCACGCGAATTGCATTAAGTGCGTTTAATGTTATTGGTGTGACACAAGAAATCGCGGAACGAAGCGTCATTGTC
AGGCAGGAGTATGGCATGAAACTCCCGGATGCGATCATTCTGGCCACTGCACAAGTCCATCGTTGTGAGCTTGTGACGCG
TAATACAAAAGACTTTGCCGGCATTCCAGGAGTGATCACGCCGTATCACTTGTAG

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT167442 WP_001307405.1 NZ_CP058780:c562803-562468 [Shigella flexneri]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT167442 NZ_CP075128:c4994101-4993859 [Salmonella enterica subsp. diarizonae serovar 60:r:e,n,x,z15]
ATGATGGCTGGGATGGATATGGGCAGAATTTTACTCGATTTATCAGACGATGTGATTAAACGGCTTGACGATCTCAAGGT
GCAACGCAACCTACCTCGTGCAGAACTCTTGCGGGAGGCTGTTGAGCAGTATCTGGAAAGACAGGATCGGGCTGAGACCA
CAATTTCGAAAGCATTGGGGCTCTGGCAAGGCTGTGAGGAAGATGGCGTTGAATACCAACGTAAACTTCGTGAGGAATGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References