Detailed information of TA system TA167437

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 107406..107660
• Replicon: plasmid unnamed1
• Accession: NZ_CP058772
• Organism: Shigella flexneri strain STLIN_11

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: HZS97_RS24215
• Protein ID: WP_001312851.1
• Coordinates: 107511..107660 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	107406..107467 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZS97_RS24180 (103056)	103056..103268	+	213	WP_001299730.1	ANR family transcriptional regulator	-
HZS97_RS24185 (103570)	103570..103659	-	90	Protein_111	IS1 family transposase	-
HZS97_RS24190 (103714)	103714..104391	+	678	WP_001339397.1	IS66-like element accessory protein TnpA	-
HZS97_RS24195 (104391)	104391..104739	+	349	Protein_113	IS66 family insertion sequence element accessory protein TnpB	-
HZS97_RS24200 (104759)	104759..106330	+	1572	WP_284527037.1	IS66 family transposase	-
HZS97_RS24205 (106368)	106368..106984	-	617	Protein_115	IS1-like element IS1A family transposase	-
HZS97_RS24210 (107085)	107085..107267	+	183	WP_000968309.1	hypothetical protein	-
- (107406)	107406..107467	-	62	NuclAT_0	-	Antitoxin
- (107406)	107406..107467	-	62	NuclAT_0	-	Antitoxin
- (107406)	107406..107467	-	62	NuclAT_0	-	Antitoxin
- (107406)	107406..107467	-	62	NuclAT_0	-	Antitoxin
HZS97_RS24215 (107511)	107511..107660	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
HZS97_RS24220 (107944)	107944..108201	+	258	WP_000083833.1	replication regulatory protein RepA	-
HZS97_RS24225 (108436)	108436..108510	+	75	WP_001365705.1	RepA leader peptide Tap	-
HZS97_RS24230 (108503)	108503..108949	+	447	Protein_120	plasmid replication initiator RepA	-
HZS97_RS24235 (108949)	108949..109563	-	615	Protein_121	VENN motif pre-toxin domain-containing protein	-
HZS97_RS24240 (110270)	110270..111489	+	1220	Protein_122	arginine deiminase	-
HZS97_RS24245 (111500)	111500..112410	+	911	Protein_123	carbamate kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaTEM-1B / aac(3)-IId / aph(3')-Ia / sul3 / lnu(F) / ant(3'')-Ia / tet(A) / sul2 / floR / dfrA12 / aadA2 / cmlA1 / tet(M) / sitABCD	iucA / iucB / iucB / iucC / iucC / iucD / iutA / iutA / iutA	1..121463	121463

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T167437 WP_001312851.1 NZ_CP058772:107511-107660 [Shigella flexneri]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T167437 NZ_CP075128:3272938-3273120 [Salmonella enterica subsp. diarizonae serovar 60:r:e,n,x,z15]
ATGAAATACAACGAGTTCAGGCGGTGGCTCATCCGACAAGGTGCAAAGTTTATCAATGCGCCAGGTGGCGGGAGTCATCA
ACGCGTCATACTAAACGGCAGGGAATCAGTATTTCCCTACCACGGCGCTAAAGAGATACCGGAGCCACTAAGAAAGAAAA
TACTCAAGGATTTGGGGCTGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT167437 NZ_CP058772:c107467-107406 [Shigella flexneri]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin