Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3817086..3817627 Replicon chromosome
Accession NZ_CP058771
Organism Shigella flexneri strain STLIN_11

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag HZS97_RS18635 Protein ID WP_000615983.1
Coordinates 3817349..3817627 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag HZS97_RS18630 Protein ID WP_000729703.1
Coordinates 3817086..3817346 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZS97_RS18610 (3812862) 3812862..3813647 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
HZS97_RS18615 (3813619) 3813619..3815331 + 1713 Protein_3649 flagellar biosynthesis protein FlhA -
HZS97_RS18620 (3815454) 3815454..3815951 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
HZS97_RS18625 (3816127) 3816127..3816876 - 750 WP_000056849.1 C40 family peptidase -
HZS97_RS18630 (3817086) 3817086..3817346 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
HZS97_RS18635 (3817349) 3817349..3817627 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
HZS97_RS18640 (3817783) 3817783..3818523 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
HZS97_RS18645 (3818494) 3818494..3819261 - 768 WP_000333380.1 class II glutamine amidotransferase -
HZS97_RS18650 (3819377) 3819377..3819955 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3816127..3827158 11031


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T167431 WP_000615983.1 NZ_CP058771:3817349-3817627 [Shigella flexneri]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T167431 NZ_CP075127:4560070-4560381 [Salmonella enterica]
GTGCATGTTATCAGCCGAAAACCGTTCAATGAAGCGATGCTCATGTATCCAAACCATGAGCTTGCTCTCACTGAGTTGCT
GAATGTTCTGGAGAAAAAAACATTCACTCAGCCTGAAGAGATGAAGCGGTATATTCCTTCACTGGATAATTTTAAGTACA
GAGATAAATGGTGGGTGATTGATGTTTCTGGTAACAGCCTGAGACTCATTTCCTACATAGATTTCAGGTTACATAAGATA
TTTGTGAAACATATCGTTTCCCATGCCGAATATGACAAACTGACCGCATATTATCGGGGTAATAAAGAATGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT167431 WP_000729703.1 NZ_CP058771:3817086-3817346 [Shigella flexneri]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT167431 NZ_CP075127:4560378-4560824 [Salmonella enterica]
ATGAGAACTCATCGTCAGATGGATGCAACCAGCGCAAAAAAGATCGTTGATACCTTCAGCGATGCGGTAAAAACCGTCCC
ACTGATGGGGGAAGACCGAAATGACAATGAGTATCGCAGGGCACTAGCGCTAGTGGAGTTTCTGGTCGACCACGACGATC
TTGAAAACCCACTATTTGAATTGCTCTGTGCCCGAATCAGTGAATACGAAAAACATGCGCCGGAATTCAAATCACTCAAC
CAACACCTGGAGAAAACACCCCCTGGCGTTTCAGTATTGCGAACGCTCATGGATCAATACGGTCTCAAAGCAGCAGATCT
TGCCAACGAACTTGGTTCTAAATCGAACGTCAGCAACATCTTAAATGGCCGCAGAGCACTAACGGTTAATCATATTAAAG
CGCTTACACAACGCTTTAAACTACCAGCGGATGCCTTCATCGAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References