Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 3570005..3570684 Replicon chromosome
Accession NZ_CP058771
Organism Shigella flexneri strain STLIN_11

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag HZS97_RS17435 Protein ID WP_284526714.1
Coordinates 3570382..3570684 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag HZS97_RS17430 Protein ID WP_000806442.1
Coordinates 3570005..3570346 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZS97_RS17420 (3566249) 3566249..3567181 - 933 WP_032258308.1 glutaminase A -
HZS97_RS17425 (3567443) 3567443..3569947 + 2505 WP_000083955.1 copper-exporting P-type ATPase CopA -
HZS97_RS17430 (3570005) 3570005..3570346 - 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
HZS97_RS17435 (3570382) 3570382..3570684 - 303 WP_284526714.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HZS97_RS17440 (3570817) 3570817..3571611 + 795 WP_000365173.1 TraB/GumN family protein -
HZS97_RS17445 (3571815) 3571815..3572294 + 480 WP_000186631.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
HZS97_RS17450 (3572331) 3572331..3573983 - 1653 WP_000771752.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
HZS97_RS17455 (3574201) 3574201..3575421 + 1221 WP_001251608.1 fosmidomycin MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(7-100)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11765.35 Da        Isoelectric Point: 10.4476

>T167428 WP_284526714.1 NZ_CP058771:c3570684-3570382 [Shigella flexneri]
MAQKNNIRSFRDARLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T167428 NZ_CP075127:c4122372-4122088 [Salmonella enterica]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGATTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGGTCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGTCTGGTGTACCAGGTTCGGGATGAAGTTGTGATTGTGTTTGTTGTCGCGGTCGGCAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT167428 WP_000806442.1 NZ_CP058771:c3570346-3570005 [Shigella flexneri]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT167428 NZ_CP075127:c4122604-4122362 [Salmonella enterica]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTATTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCACTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References