Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2341782..2342344 Replicon chromosome
Accession NZ_CP058771
Organism Shigella flexneri strain STLIN_11

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag HZS97_RS11285 Protein ID WP_000605676.1
Coordinates 2342066..2342344 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag HZS97_RS11280 Protein ID WP_000781370.1
Coordinates 2341782..2342066 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZS97_RS11265 (2337141) 2337141..2340188 + 3048 WP_014639908.1 formate dehydrogenase-N subunit alpha -
HZS97_RS11270 (2340201) 2340201..2341085 + 885 WP_001240582.1 formate dehydrogenase N subunit beta -
HZS97_RS11275 (2341078) 2341078..2341731 + 654 WP_000045648.1 formate dehydrogenase-N subunit gamma -
HZS97_RS11280 (2341782) 2341782..2342066 - 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
HZS97_RS11285 (2342066) 2342066..2342344 - 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HZS97_RS11290 (2342530) 2342530..2343540 - 1011 WP_000642433.1 alcohol dehydrogenase AdhP -
HZS97_RS11295 (2343674) 2343674..2345371 - 1698 WP_000433476.1 malate dehydrogenase -
HZS97_RS11300 (2345528) 2345528..2345665 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
HZS97_RS11305 (2345767) 2345767..2345982 - 216 WP_000495771.1 biofilm-dependent modulation protein -
HZS97_RS11310 (2346327) 2346327..2346758 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T167418 WP_000605676.1 NZ_CP058771:c2342344-2342066 [Shigella flexneri]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T167418 NZ_CP075126:38029-38445 [Salmonella enterica]
GTGAACAAAACGTATATGCTGGATACCAATATCTGCTCATTCATCATGCGTGAGCAGCCGAAGGCCGTGATCAGACGCTT
AGAGCAAGCGGTGCTGCGCAACCATCGCATCGTGGTCTCCGCTATTACCTACGCCGAAATGCGCTTCGGTGCTATCGGGA
AAAAGGCCTCGCCCCGTCATGGCCAACTGGTCGATGCGTTCTGCGCCCGCCTCGATGCGATTCTGCCGTGGGACCGTGCC
GCAGTCGATGCCACTGTAGAGGTCAAAGCAGCACTAACCGCAGCCGGTACACCCATCGGTCCCAACGACACAGCGATTGC
CGGACATGCTATAGCTGCAGGGGCCATTCTGGTGACCAATAATACGCGAGAGTTTGAGCGGGTGCCAAATTTGGTATTAG
AAGACTGGGTTAAATAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT167418 WP_000781370.1 NZ_CP058771:c2342066-2341782 [Shigella flexneri]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT167418 NZ_CP075126:37802-38032 [Salmonella enterica]
ATGCGAACAGTATCTATTTTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCTCGCGATTTGGATTTTGAAGGGGTGAG
TGAGTTGGAGATTGTCCGAGAAGGGGACAGCATCATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
AAAAGGCCGACGCAGACTTTTTGGTGGAGCGTGAAGACGTGGTCAGTGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References