Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 677717..678516 Replicon chromosome
Accession NZ_CP058771
Organism Shigella flexneri strain STLIN_11

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag HZS97_RS03320 Protein ID WP_000347273.1
Coordinates 677717..678181 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag HZS97_RS03325 Protein ID WP_001307405.1
Coordinates 678181..678516 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZS97_RS03290 (672718) 672718..673152 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
HZS97_RS03295 (673170) 673170..674048 - 879 WP_001300474.1 PTS N-acetylgalactosamine transporter subunit IID -
HZS97_RS03300 (674038) 674038..674817 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
HZS97_RS03305 (674828) 674828..675301 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
HZS97_RS03310 (675324) 675324..676604 - 1281 WP_000681903.1 tagatose-bisphosphate aldolase subunit KbaZ -
HZS97_RS03315 (676853) 676853..677662 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
HZS97_RS03320 (677717) 677717..678181 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
HZS97_RS03325 (678181) 678181..678516 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
HZS97_RS03330 (678665) 678665..680236 - 1572 WP_001723936.1 galactarate dehydratase -
HZS97_RS03335 (680611) 680611..681945 + 1335 WP_001723935.1 galactarate/glucarate/glycerate transporter GarP -
HZS97_RS03340 (681961) 681961..682731 + 771 WP_001723934.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 677717..688995 11278


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T167412 WP_000347273.1 NZ_CP058771:c678181-677717 [Shigella flexneri]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T167412 NZ_CP075125:c2179605-2179423 [Salmonella enterica]
TTGAAAAGCTCGGAGTTGATAAAACTACTGGAAAGCAAAGGGTGGACGCTGGAACGAATTAACGGTAGTCATCACCATTT
TTCGCACCCTGACTTTCCTGTGATTATTACGGTTCCTCATCCCAGGAAAGATATAAAACCCGGAACGCTTAACCAGATAC
TGAAAAAGGCAAAGCTAAAGTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT167412 WP_001307405.1 NZ_CP058771:c678516-678181 [Shigella flexneri]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT167412 NZ_CP075125:c2179380-2178967 [Salmonella enterica]
ATGTTGTATCCTGCTTTTATCGAAGTGGATAAAGACGGTAGCGCCAGCGGGTGGTTTCCCGATGTTCCCGGCTGTATTTT
CGCCGGCAGCACTATTGAGGAAACCTATGCCGACGCACGCAGCGCTATCTGTGCTCATTTTGAAGTGCTCTGTGATAAGG
GGTTTGATATTCCCTCTCCCCGCAGCATCGAGCAACATGTCGCCGCCGAACCAGACGATTACAGTAACGGACGCTGGTTG
TATGTGGACGTAAATATGGATCAGTTTGACGGGAGAGCTGAACGCATCAACGTCACTCTTCCCCATCGCCTGCTCGATCG
CATCGACGCCACGGTAAAACAGCATCCCGACTACGGTAGCCGGAGTGCATTTCTGGCAGCAGCTGCGCGTAATGAGTTAC
AGAAATCGCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References