Detailed information of TA system TA167412

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 677717..678516
• Replicon: chromosome
• Accession: NZ_CP058771
• Organism: Shigella flexneri strain STLIN_11

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: HZS97_RS03320
• Protein ID: WP_000347273.1
• Coordinates: 677717..678181 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1EB98
• Locus tag: HZS97_RS03325
• Protein ID: WP_001307405.1
• Coordinates: 678181..678516 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZS97_RS03290 (672718)	672718..673152	-	435	WP_000948824.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-
HZS97_RS03295 (673170)	673170..674048	-	879	WP_001300474.1	PTS N-acetylgalactosamine transporter subunit IID	-
HZS97_RS03300 (674038)	674038..674817	-	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
HZS97_RS03305 (674828)	674828..675301	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
HZS97_RS03310 (675324)	675324..676604	-	1281	WP_000681903.1	tagatose-bisphosphate aldolase subunit KbaZ	-
HZS97_RS03315 (676853)	676853..677662	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
HZS97_RS03320 (677717)	677717..678181	-	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
HZS97_RS03325 (678181)	678181..678516	-	336	WP_001307405.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
HZS97_RS03330 (678665)	678665..680236	-	1572	WP_001723936.1	galactarate dehydratase	-
HZS97_RS03335 (680611)	680611..681945	+	1335	WP_001723935.1	galactarate/glucarate/glycerate transporter GarP	-
HZS97_RS03340 (681961)	681961..682731	+	771	WP_001723934.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	677717..688995	11278

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T167412 WP_000347273.1 NZ_CP058771:c678181-677717 [Shigella flexneri]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T167412 NZ_CP075125:c2179605-2179423 [Salmonella enterica]
TTGAAAAGCTCGGAGTTGATAAAACTACTGGAAAGCAAAGGGTGGACGCTGGAACGAATTAACGGTAGTCATCACCATTT
TTCGCACCCTGACTTTCCTGTGATTATTACGGTTCCTCATCCCAGGAAAGATATAAAACCCGGAACGCTTAACCAGATAC
TGAAAAAGGCAAAGCTAAAGTAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12358.94 Da
• Isoelectric Point: 4.8616

>AT167412 WP_001307405.1 NZ_CP058771:c678516-678181 [Shigella flexneri]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT167412 NZ_CP075125:c2179380-2178967 [Salmonella enterica]
ATGTTGTATCCTGCTTTTATCGAAGTGGATAAAGACGGTAGCGCCAGCGGGTGGTTTCCCGATGTTCCCGGCTGTATTTT
CGCCGGCAGCACTATTGAGGAAACCTATGCCGACGCACGCAGCGCTATCTGTGCTCATTTTGAAGTGCTCTGTGATAAGG
GGTTTGATATTCCCTCTCCCCGCAGCATCGAGCAACATGTCGCCGCCGAACCAGACGATTACAGTAACGGACGCTGGTTG
TATGTGGACGTAAATATGGATCAGTTTGACGGGAGAGCTGAACGCATCAACGTCACTCTTCCCCATCGCCTGCTCGATCG
CATCGACGCCACGGTAAAACAGCATCCCGACTACGGTAGCCGGAGTGCATTTCTGGCAGCAGCTGCGCGTAATGAGTTAC
AGAAATCGCTTTAA

Structures

Toxin

• AlphaFold DB: V0SSH7

Antitoxin

• AlphaFold DB: A0A0E0XVC7