Detailed information of TA system TA167409

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 5022..5674
• Replicon: plasmid unnamed4
• Accession: NZ_CP058762
• Organism: Klebsiella pneumoniae strain STLIN_3

Toxin (Protein)

• Gene name: higB
• Uniprot ID: B2CBE9
• Locus tag: HZT06_RS26970
• Protein ID: WP_012457127.1
• Coordinates: 5324..5674 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: B2CBE8
• Locus tag: HZT06_RS26965
• Protein ID: WP_012457126.1
• Coordinates: 5022..5321 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZT06_RS26940 (HZT06_26885)	1058..1813	+	756	WP_023316386.1	MobC family replication-relaxation protein	-
HZT06_RS26945 (HZT06_26890)	1916..2446	+	531	WP_012457122.1	phospholipase D family protein	-
HZT06_RS26950 (HZT06_26895)	2630..3598	-	969	WP_012457123.1	zincin-like metallopeptidase domain-containing protein	-
HZT06_RS26955 (HZT06_26900)	3983..4648	+	666	WP_094309421.1	hypothetical protein	-
HZT06_RS26960 (HZT06_26905)	4811..4996	+	186	WP_117061473.1	hypothetical protein	-
HZT06_RS26965 (HZT06_26910)	5022..5321	-	300	WP_012457126.1	helix-turn-helix domain-containing protein	Antitoxin
HZT06_RS26970 (HZT06_26915)	5324..5674	-	351	WP_012457127.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HZT06_RS26975 (HZT06_26920)	6068..6685	+	618	WP_012457128.1	ParA family protein	-
HZT06_RS26980 (HZT06_26925)	6740..6979	+	240	WP_012457129.1	plasmid partition protein ParG	-
HZT06_RS26985 (HZT06_26930)	7419..8675	-	1257	WP_117061474.1	GIY-YIG nuclease family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..30500	30500

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-107)

Antitoxin

• Cro/C1-type helix-turn-helix domain (34-83)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13378.26 Da
• Isoelectric Point: 6.7130

>T167409 WP_012457127.1 NZ_CP058762:c5674-5324 [Klebsiella pneumoniae]
MWDIITTECFDGWFLAQSDALRESVYEAMGVLEKFGPALGRPYVDTLNDSDFANMKELRIQHHGNPVRAFFAFDPTRRAI
VLCAGDKTGANEKRFYKDMIRLADSEYRKHLAKLEK

Nucleotide

• Length: 351 bp

>T167409 NZ_CP075125:937694-938107 [Salmonella enterica]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGCCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCGTCCGCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CGCGCCAACACTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11011.75 Da
• Isoelectric Point: 5.8719

>AT167409 WP_012457126.1 NZ_CP058762:c5321-5022 [Klebsiella pneumoniae]
MATYRELLAKESPEMQARVAERVEEVSIQIALSMLRDELNISQTELAAVMGVKQPSVARMEQADNDPRLSTLKRYVKALG
GELSLDVTLPTGKRVAFHI

Nucleotide

• Length: 300 bp

>AT167409 NZ_CP075125:937447-937713 [Salmonella enterica]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGATGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: B2CBE9

Antitoxin

• AlphaFold DB: B2CBE8