Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 115832..116559 Replicon plasmid unnamed2
Accession NZ_CP058760
Organism Klebsiella pneumoniae strain STLIN_3

Toxin (Protein)


Gene name higB Uniprot ID A0A663AUB1
Locus tag HZT06_RS26530 Protein ID WP_000558568.1
Coordinates 115832..116143 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID V0AHC4
Locus tag HZT06_RS26535 Protein ID WP_000990392.1
Coordinates 116140..116559 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT06_RS26510 (HZT06_26450) 112346..113065 + 720 WP_001102107.1 (Fe-S)-binding protein -
HZT06_RS26515 (HZT06_26455) 113076..114503 + 1428 WP_000023895.1 LutB/LldF family L-lactate oxidation iron-sulfur protein -
HZT06_RS26520 (HZT06_26460) 114496..115191 + 696 WP_000370412.1 lactate utilization protein C -
HZT06_RS26525 (HZT06_26465) 115241..115621 - 381 Protein_112 gluconate permease -
HZT06_RS26530 (HZT06_26470) 115832..116143 + 312 WP_000558568.1 type II toxin-antitoxin system HigB family toxin Toxin
HZT06_RS26535 (HZT06_26475) 116140..116559 + 420 WP_000990392.1 helix-turn-helix domain-containing protein Antitoxin
HZT06_RS26540 (HZT06_26480) 116596..117798 - 1203 WP_003031541.1 hypothetical protein -
HZT06_RS26545 (HZT06_26485) 117791..118120 - 330 WP_000121260.1 hypothetical protein -
HZT06_RS26550 (HZT06_26490) 118117..118776 - 660 WP_176759338.1 maturation control protein -
HZT06_RS26555 (HZT06_26495) 119179..119292 - 114 Protein_118 IS1 family transposase -
HZT06_RS26560 (HZT06_26500) 119429..119752 + 324 WP_157843664.1 Tn7-like element transposition protein TnsE -
HZT06_RS26565 (HZT06_26505) 119916..120824 + 909 WP_117250874.1 WYL domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - mrkA / mrkB / mrkC / mrkF / mrkJ 1..135057 135057


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(83-136)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12372.11 Da        Isoelectric Point: 10.0935

>T167408 WP_000558568.1 NZ_CP058760:115832-116143 [Klebsiella pneumoniae]
MHVVSRAPFDTATRQFPNQAAALDDVYRTLKRENYTSPDEMKKRFASLDRMKYREKWWVIDVGGGNLRVMFFADFERGKI
FIKHITTHAEYDKLTDFYRRTKE

Download         Length: 312 bp

>T167408 NZ_CP075125:201635-202120 [Salmonella enterica]
GTGGGACGTGTAACAGCACCAGAACCTTTGTCCGCTTTTCATCAGGTAGCTGAGTTCGTCAGCGGTGAAGCTGTGCTCGA
TGACTGGTTGAAGCAAAAGGGCCTCAAAAACCAGGCTCTCGGAGCGGCCAGAACATTTGTGGTGTGCAAGAAAGACACGA
AGCAAGTAGCCGGTTTTTACTCTCTGGCCACCGGTAGCGTCAACCATACAGAAGCGACAGGCAACCTTCGGCGTAACATG
CCAGATCCCATCCCTGTCATTATACTTGCCCGTCTTGCTGTCGATCTCTCATTCCATGGAAAAGGGCTTGGTGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTTACCG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAATCATCACAAACTCAGCAGCGAACTTTGTTCCTTAGGCTCCCT
CAATAG

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15534.71 Da        Isoelectric Point: 4.4702

>AT167408 WP_000990392.1 NZ_CP058760:116140-116559 [Klebsiella pneumoniae]
MMYTDAIQAANSLVSIVPLLGGNASRKDYEDALTLVEYLVEHEPDHPLVDMLVAKIAQYEDEAEEFAEFNDRIAALPSGV
ALLRVLMDQHKLTQSDFEEEIGKKSLVSRILNGTRSLTLDHMKALARRFNIPPSSFMDA

Download         Length: 420 bp

>AT167408 NZ_CP075125:201360-201647 [Salmonella enterica]
ATGCTATACAAGGGGTGTCTCATGAAATCAGATGTTCAACTTAACCTTAGAGCTAAGGAATCGCAGCGGGCGCTCATTGA
TGCAGCTGCGGAAATCCTTCACAAGTCACGTACAGATTTCATTCTGGAAACGGCCTGCCAGGCTGCCGAGAAAGTGATCC
TTGACCGCCGTGTATTTAACTTTAACGATGAGCAATATGAGGAGTTCATCAATCTGCTTGATGCACCGGTCGCAGATGAT
CCCGTTATCGAAAAACTGCTGGCAAGGAAACCTCAGTGGGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AUB1

Antitoxin

Source ID Structure
AlphaFold DB V0AHC4

References