Detailed information of TA system TA167361

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 120356..120610
• Replicon: plasmid unnamed1
• Accession: NZ_CP058749
• Organism: Escherichia coli strain STLIN_13

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: HZS99_RS24020
• Protein ID: WP_001312851.1
• Coordinates: 120461..120610 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	120356..120417 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZS99_RS23985 (116008)	116008..116220	+	213	WP_005012601.1	hypothetical protein	-
HZS99_RS23990 (116521)	116521..116610	-	90	Protein_128	IS1 family transposase	-
HZS99_RS23995 (116665)	116665..117342	+	678	WP_001339397.1	IS66-like element accessory protein TnpA	-
HZS99_RS24000 (117342)	117342..117689	+	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
HZS99_RS24005 (117709)	117709..119280	+	1572	WP_023149734.1	IS66-like element ISCro1 family transposase	-
HZS99_RS24010 (119318)	119318..119934	-	617	Protein_132	IS1-like element IS1A family transposase	-
HZS99_RS24015 (120035)	120035..120217	+	183	WP_000968309.1	hypothetical protein	-
- (120356)	120356..120417	-	62	NuclAT_0	-	Antitoxin
- (120356)	120356..120417	-	62	NuclAT_0	-	Antitoxin
- (120356)	120356..120417	-	62	NuclAT_0	-	Antitoxin
- (120356)	120356..120417	-	62	NuclAT_0	-	Antitoxin
HZS99_RS24020 (120461)	120461..120610	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
HZS99_RS24025 (120894)	120894..121151	+	258	WP_000083833.1	replication regulatory protein RepA	-
HZS99_RS24030 (121256)	121256..121441	+	186	WP_077818652.1	protein CopA/IncA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaTEM-1B / aac(3)-IId / aph(3')-Ia / sul3 / lnu(F) / ant(3'')-Ia / tet(A) / sul2 / floR / dfrA12 / aadA2 / cmlA1 / tet(M) / sitABCD	iucA / iucB / iucC / iucD / iutA	1..121453	121453

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T167361 WP_001312851.1 NZ_CP058749:120461-120610 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T167361 NZ_CP075118:c989176-988649 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGATGTTTACAGACTGGCATGAGGCCGCGATAGGGAAAACCCACAATCGAATGAATTTTGATTGTGGGGATGCCGATCT
GAACCAATTCCTGCAACGCCATGCACGACAAAATCATGAGAAAGGGACAACGAAAACCTATGTTGCGCTTGATAATTCGG
ATGTTACACGTATCCACGGCTTTTACTCAGTCAGTCCTGCATCACTGATATATGCACAGGTTCCCGGCGCAATCAGCAAA
GGATTAGGGAGATACGATGTGCCAGTGTTTCGTCTGGGTCGTTTAGCCGTAGACAAATCTATGCAGGGGCAAGGGCTGGG
AGCACAACTTTTGTTATCTGCCGGAAAGCGTTGCATACAGGCGGCTTTGCAGGTCGGTGGCGTAGCCCTACTTATTGATG
CCAAAAATAAACAGGTCTGCGACTGGTACAAAGGATTTGGCGCAGTACCATTAAACGATCAACCCCTTTCCTTGTTGCTG
TCGTTTAAAACGCTTTATGCTGCTTTATCTGCATCTGGTAGGTTATGA

Antitoxin

Nucleotide

• Length: 62 bp

>AT167361 NZ_CP058749:c120417-120356 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin