Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2341357..2341919 Replicon chromosome
Accession NZ_CP058748
Organism Escherichia coli strain STLIN_13

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag HZS99_RS11200 Protein ID WP_000605676.1
Coordinates 2341641..2341919 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag HZS99_RS11195 Protein ID WP_000781370.1
Coordinates 2341357..2341641 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZS99_RS11180 (2336716) 2336716..2339763 + 3048 WP_012311994.1 formate dehydrogenase-N subunit alpha -
HZS99_RS11185 (2339776) 2339776..2340660 + 885 WP_001240582.1 formate dehydrogenase N subunit beta -
HZS99_RS11190 (2340653) 2340653..2341306 + 654 WP_000045650.1 formate dehydrogenase-N subunit gamma -
HZS99_RS11195 (2341357) 2341357..2341641 - 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
HZS99_RS11200 (2341641) 2341641..2341919 - 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HZS99_RS11205 (2342105) 2342105..2343115 - 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
HZS99_RS11210 (2343249) 2343249..2344946 - 1698 WP_000433464.1 malate dehydrogenase -
HZS99_RS11215 (2345103) 2345103..2345240 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
HZS99_RS11220 (2345342) 2345342..2345557 - 216 WP_000495766.1 biofilm-dependent modulation protein -
HZS99_RS11225 (2345902) 2345902..2346333 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T167341 WP_000605676.1 NZ_CP058748:c2341919-2341641 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T167341 NZ_CP075115:4382007-4382318 [Salmonella enterica subsp. enterica serovar Eastbourne]
GTGCATGTTATCAGCCGAAAACCGTTCAATGAAGCGATGCTCATGTATCCAAACCATGAGCTTGCTCTCACTGAGTTGCT
GAATGTTCTGGAGAAAAAAACATTCACTCAGCCTGAAGAGATGAAGCGGTATATTCCTTCACTGGATAATTTTAAGTACA
GAGATAAATGGTGGGTGATTGATGTTTCTGGTAACAGCCTGAGACTCATTTCCTACATAGATTTCAGGTTACATAAGATA
TTTGTGAAACATATCGTTTCCCATGCCGAATATGACAAACTGACCGCATATTATCGGGGTAATAAAGAATGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT167341 WP_000781370.1 NZ_CP058748:c2341641-2341357 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT167341 NZ_CP075115:4382315-4382761 [Salmonella enterica subsp. enterica serovar Eastbourne]
ATGAGAACTCATCGTCAGATGGATGCAACCAGCGCAAAAAAGATCGTTGATACCTTCAGCGATGCGGTAAAAACCGTCCC
ACTGATGGGGGAAGACCGAAATGACAATGAGTATCGCAGGGCACTAGCGCTAGTGGAGTTTCTGGTCGACCACGACGATC
TTGAAAACCCACTATTTGAATTGCTCTGTGCCCGAATCAGTGAATACGAAAAACATGCGCCGGAATTCAAAGCACTCAAC
CAACACCTGGAGAAAACGCCCCCTGGCGTTTCAGTATTGCGAACGCTCATGGATCAATACGGTCTCAAAGCAGCAGATCT
TGCCAACGAACTTGGTTCTAAATCGAACGTCAGCAACATCTTAAATGGCCGCAGAGCACTAACGGTTAATCATATTAAAG
CGCTTACACAACGCTTTAAACTACCAGCGGATGCCTTCATCGAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References