Detailed information of TA system TA167335

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 677213..678012
• Replicon: chromosome
• Accession: NZ_CP058748
• Organism: Escherichia coli strain STLIN_13

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: HZS99_RS03295
• Protein ID: WP_000347273.1
• Coordinates: 677213..677677 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: D6JF08
• Locus tag: HZS99_RS03300
• Protein ID: WP_001308975.1
• Coordinates: 677677..678012 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZS99_RS03265 (672214)	672214..672648	-	435	WP_000948834.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-
HZS99_RS03270 (672666)	672666..673544	-	879	WP_001298314.1	PTS N-acetylgalactosamine transporter subunit IID	-
HZS99_RS03275 (673534)	673534..674313	-	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
HZS99_RS03280 (674324)	674324..674797	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
HZS99_RS03285 (674820)	674820..676100	-	1281	WP_000681961.1	tagatose-bisphosphate aldolase subunit KbaZ	-
HZS99_RS03290 (676349)	676349..677158	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
HZS99_RS03295 (677213)	677213..677677	-	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
HZS99_RS03300 (677677)	677677..678012	-	336	WP_001308975.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
HZS99_RS03305 (678161)	678161..679732	-	1572	WP_001273756.1	galactarate dehydratase	-
HZS99_RS03310 (680107)	680107..681441	+	1335	WP_000599651.1	galactarate/glucarate/glycerate transporter GarP	-
HZS99_RS03315 (681457)	681457..682227	+	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T167335 WP_000347273.1 NZ_CP058748:c677677-677213 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T167335 NZ_CP075115:2274528-2274812 [Salmonella enterica subsp. enterica serovar Eastbourne]
ATGACTTATAAGCTGGCATTTAACGAATCAGCACTAAAAGAGTGGAAAAAACTGGGTCATACCATACAGGAACAGTTTAA
AAAAAAGCTAAGGGAACGGCTTGAAAACCCTCGGGTTCCGGCTTCTCAATTACATGGAAGAAAGGATCAGTACAAAATTA
AGCTGCGTGGTGCAGGATACAGACTGGTGTACAGCGTCGAAGATGAAATTATCACGGTAACTGTCATTGGTGTTGGAAAG
CGTGAAAATGATGCCGTCTACAAAGTGACACAACACAGAAGCTAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12343.97 Da
• Isoelectric Point: 4.8616

>AT167335 WP_001308975.1 NZ_CP058748:c678012-677677 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT167335 NZ_CP075115:2274290-2274538 [Salmonella enterica subsp. enterica serovar Eastbourne]
ATGGCATTTCAAATTTTAACGACTACTGCAGCAAGCATTACAGAACTGAAACGTGATCCAATGGGAACTTTCAATGCCGG
AGATGGCGCTCCAGTTGCCATTCTTAACCGTAATGAACCGGCTTTCTACTGTGTGCCTCCGGCACTTTATGCTCATCTTA
TGGATATTCTTGAGGATGAAGAACTCGGTCGTATTATCGATGAGCGTGCGAATGAACGCGTCATTGAGGTCAACATTGAT
GACTTATAA

Structures

Toxin

• AlphaFold DB: V0SSH7

Antitoxin

• AlphaFold DB: D6JF08