Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 2129913..2130454 Replicon chromosome
Accession NZ_CP058725
Organism Escherichia coli strain S171-1

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag HZS87_RS10545 Protein ID WP_000615976.1
Coordinates 2130176..2130454 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag HZS87_RS10540 Protein ID WP_000729704.1
Coordinates 2129913..2130173 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZS87_RS10520 (2125588) 2125588..2126373 - 786 WP_000207587.1 putative lateral flagellar export/assembly protein LafU -
HZS87_RS10525 (2126345) 2126345..2128057 + 1713 Protein_2077 flagellar biosynthesis protein FlhA -
HZS87_RS10530 (2128281) 2128281..2128778 - 498 WP_089615104.1 REP-associated tyrosine transposase RayT -
HZS87_RS10535 (2128954) 2128954..2129727 - 774 WP_015675129.1 C40 family peptidase -
HZS87_RS10540 (2129913) 2129913..2130173 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
HZS87_RS10545 (2130176) 2130176..2130454 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
HZS87_RS10550 (2130610) 2130610..2131350 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
HZS87_RS10555 (2131321) 2131321..2132088 - 768 WP_000333379.1 class II glutamine amidotransferase -
HZS87_RS10560 (2132294) 2132294..2132872 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 2122755..2140708 17953


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T167283 WP_000615976.1 NZ_CP058725:2130176-2130454 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T167283 NZ_CP075109:c4152262-4151978 [Salmonella enterica]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGATCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGCTTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTCGCGGTCGGTAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT167283 WP_000729704.1 NZ_CP058725:2129913-2130173 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT167283 NZ_CP075109:c4152494-4152252 [Salmonella enterica]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCATTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References