Detailed information of TA system TA167282

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 2123716..2124410
• Replicon: chromosome
• Accession: NZ_CP058725
• Organism: Escherichia coli strain S171-1

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: HZS87_RS10505
• Protein ID: WP_001263493.1
• Coordinates: 2123716..2124114 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: HZS87_RS10510
• Protein ID: WP_000554757.1
• Coordinates: 2124117..2124410 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (2119377)	2119377..2119457	-	81	NuclAT_9	-	-
- (2119377)	2119377..2119457	-	81	NuclAT_9	-	-
- (2119377)	2119377..2119457	-	81	NuclAT_9	-	-
- (2119377)	2119377..2119457	-	81	NuclAT_9	-	-
HZS87_RS10475 (2118717)	2118717..2119961	-	1245	WP_000189541.1	esterase FrsA	-
HZS87_RS10480 (2120053)	2120053..2120511	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
HZS87_RS10485 (2120772)	2120772..2122229	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
HZS87_RS10490 (2122286)	2122286..2122807	-	522	Protein_2070	peptide chain release factor H	-
HZS87_RS10495 (2122806)	2122806..2123009	-	204	Protein_2071	RtcB family protein	-
HZS87_RS10500 (2123254)	2123254..2123706	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
HZS87_RS10505 (2123716)	2123716..2124114	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
HZS87_RS10510 (2124117)	2124117..2124410	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
HZS87_RS10515 (2124462)	2124462..2125517	-	1056	WP_001226164.1	DNA polymerase IV	-
HZS87_RS10520 (2125588)	2125588..2126373	-	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
HZS87_RS10525 (2126345)	2126345..2128057	+	1713	Protein_2077	flagellar biosynthesis protein FlhA	-
HZS87_RS10530 (2128281)	2128281..2128778	-	498	WP_089615104.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA	2122755..2140708	17953

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T167282 WP_001263493.1 NZ_CP058725:c2124114-2123716 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T167282 NZ_CP075109:3639414-3639671 [Salmonella enterica]
ATGAATGCCTCCGGCGTCAGCGTCCGCCACATCAACAGCAAAACCCACATGACCACCTATTACTCACAAATCCCCAGCCT
GCACCTTAAGGGTGACTGGCTGGAAGAAGCGGGATTTGAGACCGGGCGCGGCGTGACCGTGAAGATTTCACAGGGGTGTA
TTGTGCTGATGGCGGACAGTAACGAGGAGCAGAAGCTGCGCGAACAGCTTTACAAGGCTGAACAGGTGGTTAAGGGGATG
CGGGACGTGATTGTTTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT167282 WP_000554757.1 NZ_CP058725:c2124410-2124117 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT167282 NZ_CP075109:c3639589-3639321 [Salmonella enterica]
TCCGCCATCAGCACAATACACCCCTGTGAAATCTTCACGGTCACGCCGCGCCCGGTCTCAAATCCCGCTTCTTCCAGCCA
GTCACCCTTAAGGTGCAGGCTGGGGATTTGTGAGTAATAGGTGGTCATGTGGGTTTTGCTGTTGATGTGGCGGACGCTGA
CGCCGGAGGCATTCATATTTGCTGACTAAATAAATTCTTAATTATCCGCCGGATGCTGGCTGATTGTGGAGCTCAGGGTG
AGCGAGTATGAGCGCGACAGCCTGCACCG

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6