Detailed information of TA system TA167245

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 428678..429405
• Replicon: chromosome
• Accession: NZ_CP058719
• Organism: Escherichia coli strain M159-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: HZS86_RS02070
• Protein ID: WP_130062357.1
• Coordinates: 429091..429405 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HZS86_RS02065
• Protein ID: WP_000560266.1
• Coordinates: 428678..429094 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZS86_RS02055 (423838)	423838..426189	+	2352	WP_130062358.1	alpha-glucosidase	-
HZS86_RS02060 (426615)	426615..428633	+	2019	WP_000121433.1	NADPH-dependent 2,4-dienoyl-CoA reductase	-
HZS86_RS02065 (428678)	428678..429094	-	417	WP_000560266.1	type II toxin-antitoxin system antitoxin HigA	Antitoxin
HZS86_RS02070 (429091)	429091..429405	-	315	WP_130062357.1	type II toxin-antitoxin system toxin HigB	Toxin
HZS86_RS02075 (429689)	429689..430825	-	1137	WP_000018695.1	23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG	-
HZS86_RS02080 (430910)	430910..431413	+	504	WP_001333820.1	M48 family metallopeptidase	-
HZS86_RS02085 (431490)	431490..432182	+	693	WP_000942548.1	vancomycin high temperature exclusion protein	-
HZS86_RS02090 (432261)	432261..433247	+	987	WP_000617698.1	Gfo/Idh/MocA family oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-135)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12129.14 Da
• Isoelectric Point: 9.9845

>T167245 WP_130062357.1 NZ_CP058719:c429405-429091 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKYYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Nucleotide

• Length: 315 bp

>T167245 NZ_CP075106:2430233-2430517 [Salmonella enterica]
ATGACTTATAAGCTGGCATTTAACGAATCTGCACTAAAAGAGTGGAAAAAGCTGGGACATACTATACAGGAACAGTTTAA
AAAAAAGTTACGGGAACGGCTTGAAAACCCTCGGGTTCCGGCTTCTCAATTACATGGAAGAAAGGATCAGTACAAAATTA
AGCTGCGTGGTGCAGGATACAGACTGGTGTACAGCGTCGAAGATGAAATTATCACGGTAACTGTCATTGGCGTTGGAAAG
CGTGAAAATGATGCCATCTACAAAGTGACACGACACAGAAGCTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 14995.42 Da
• Isoelectric Point: 4.4547

>AT167245 WP_000560266.1 NZ_CP058719:c429094-428678 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Nucleotide

• Length: 417 bp

>AT167245 NZ_CP075106:2429995-2430243 [Salmonella enterica]
ATGGCATTTCAAATTTTAACGACTACTGCAGCAAGCATTACTGAACTGAAACGTGACCCAATGGGAACTTTCAATGCCGG
AGATGGCGCTCCAGTTGCCATTCTTAACCGTAATGAACCGGCTTTCTACTGTGTGCCTCCGGCACTTTATGCTCATCTTA
TGGATATTCTTGAGGATGAAGAACTCGGTCGTATTATCGATGAGCGTGCGAATGAACGCGTCATTGAGGTCAACATTGAT
GACTTATAA