Detailed information of TA system TA167214

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 2651895..2652694
• Replicon: chromosome
• Accession: NZ_CP058714
• Organism: Escherichia coli strain M172-1

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: HYI45_RS12765
• Protein ID: WP_000347273.1
• Coordinates: 2651895..2652359 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: V0TI39
• Locus tag: HYI45_RS12770
• Protein ID: WP_021547164.1
• Coordinates: 2652359..2652694 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HYI45_RS12735 (2646896)	2646896..2647330	-	435	WP_000948834.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-
HYI45_RS12740 (2647348)	2647348..2648226	-	879	WP_001295548.1	PTS N-acetylgalactosamine transporter subunit IID	-
HYI45_RS12745 (2648216)	2648216..2648995	-	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
HYI45_RS12750 (2649006)	2649006..2649479	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
HYI45_RS12755 (2649502)	2649502..2650782	-	1281	WP_000681958.1	tagatose-bisphosphate aldolase subunit KbaZ	-
HYI45_RS12760 (2651031)	2651031..2651840	+	810	WP_000072171.1	aga operon transcriptional regulator AgaR	-
HYI45_RS12765 (2651895)	2651895..2652359	-	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
HYI45_RS12770 (2652359)	2652359..2652694	-	336	WP_021547164.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
HYI45_RS12775 (2652843)	2652843..2654414	-	1572	WP_001273756.1	galactarate dehydratase	-
HYI45_RS12780 (2654789)	2654789..2656123	+	1335	WP_000599651.1	galactarate/glucarate/glycerate transporter GarP	-
HYI45_RS12785 (2656139)	2656139..2656909	+	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T167214 WP_000347273.1 NZ_CP058714:c2652359-2651895 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T167214 NZ_CP075062:c819504-819127 [Escherichia coli]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGTTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCAGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12309.86 Da
• Isoelectric Point: 4.5392

>AT167214 WP_021547164.1 NZ_CP058714:c2652694-2652359 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDTQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT167214 NZ_CP075062:c819925-819551 [Escherichia coli]
GTGTCAGACACTCTCCCCGGGACAACGCATCCCGACGATAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTTGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA

Structures

Toxin

• AlphaFold DB: V0SSH7

Antitoxin

• AlphaFold DB: V0TI39