Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3518590..3519317 Replicon chromosome
Accession NZ_CP058709
Organism Escherichia coli strain S163-2

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag HZS88_RS17595 Protein ID WP_000550189.1
Coordinates 3518590..3518904 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag HZS88_RS17600 Protein ID WP_000560266.1
Coordinates 3518901..3519317 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZS88_RS17575 (3514748) 3514748..3515734 - 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -
HZS88_RS17580 (3515813) 3515813..3516505 - 693 WP_000942548.1 vancomycin high temperature exclusion protein -
HZS88_RS17585 (3516582) 3516582..3517085 - 504 WP_001333820.1 M48 family metallopeptidase -
HZS88_RS17590 (3517170) 3517170..3518306 + 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
HZS88_RS17595 (3518590) 3518590..3518904 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
HZS88_RS17600 (3518901) 3518901..3519317 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
HZS88_RS17605 (3519362) 3519362..3521380 - 2019 WP_239804017.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
HZS88_RS17610 (3521806) 3521806..3524157 - 2352 WP_000695487.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T167197 WP_000550189.1 NZ_CP058709:3518590-3518904 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T167197 NZ_CP075061:419782-419964 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTTGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTACGAGCTGTAG

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT167197 WP_000560266.1 NZ_CP058709:3518901-3519317 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT167197 NZ_CP075061:419458-419754 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References