Detailed information of TA system TA167187

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 193380..193751
• Replicon: chromosome
• Accession: NZ_CP058709
• Organism: Escherichia coli strain S163-2

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A368IS51
• Locus tag: HZS88_RS01025
• Protein ID: WP_071780718.1
• Coordinates: 193557..193751 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	193380..193558 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZS88_RS00985 (188822)	188822..189478	-	657	WP_063121046.1	carbon-nitrogen hydrolase family protein YobB	-
HZS88_RS00990 (189580)	189580..189810	-	231	WP_000916763.1	DNA polymerase III subunit theta	-
HZS88_RS00995 (189949)	189949..190323	+	375	WP_000168747.1	CopC domain-containing protein YobA	-
HZS88_RS01000 (190327)	190327..191199	+	873	WP_000879295.1	copper homeostasis membrane protein CopD	-
HZS88_RS01005 (191212)	191212..191553	+	342	WP_000976472.1	YebY family protein	-
HZS88_RS01010 (191946)	191946..193022	-	1077	WP_087891211.1	phage integrase Arm DNA-binding domain-containing protein	-
HZS88_RS01015 (192988)	192988..193269	-	282	WP_001311878.1	excisionase	-
- (193380)	193380..193558	+	179	NuclAT_0	-	Antitoxin
- (193380)	193380..193558	+	179	NuclAT_0	-	Antitoxin
- (193380)	193380..193558	+	179	NuclAT_0	-	Antitoxin
- (193380)	193380..193558	+	179	NuclAT_0	-	Antitoxin
HZS88_RS01020 (193376)	193376..193564	-	189	WP_001356607.1	DUF1187 family protein	-
HZS88_RS01025 (193557)	193557..193751	-	195	WP_071780718.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
HZS88_RS01030 (193815)	193815..194867	-	1053	WP_001004419.1	RecT family recombinase	-
HZS88_RS01035 (194879)	194879..198025	-	3147	WP_239804042.1	exodeoxyribonuclease VIII	-
HZS88_RS01040 (198050)	198050..198346	-	297	WP_000022247.1	hypothetical protein	-
HZS88_RS01045 (198421)	198421..198591	-	171	WP_112928455.1	YdaE family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	186079..249904	63825

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.97 Da
• Isoelectric Point: 9.6161

>T167187 WP_071780718.1 NZ_CP058709:c193751-193557 [Escherichia coli]
MRYEKVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYSGSEKEALARWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T167187 NZ_CP075055:c1275514-1274768 [Bacillus velezensis]
ATGCTTATGTTTTTTCAGATTATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTACTGGGCTCCATGGTCTATTGGA
CATATGATCCTGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCCGCTTATGCCGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCGGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAGAACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT167187 NZ_CP058709:193380-193558 [Escherichia coli]
GAGGACAGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTCCCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAATTTAGATTTTGAATATCTTGTCCAGTTAGTAGGTGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A368IS51

Antitoxin