Detailed information of TA system TA167167

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 5500542..5501144
• Replicon: chromosome
• Accession: NZ_CP058682
• Organism: Escherichia coli strain MBT-5

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: HZT35_RS27985
• Protein ID: WP_000897305.1
• Coordinates: 5500833..5501144 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HZT35_RS27980
• Protein ID: WP_000356397.1
• Coordinates: 5500542..5500832 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZT35_RS27955	5496088..5496723	+	636	WP_000829008.1	formate dehydrogenase cytochrome b556 subunit	-
HZT35_RS27960	5496720..5497649	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
HZT35_RS27965	5498049..5499029	+	981	WP_000399648.1	IS110-like element IS621 family transposase	-
HZT35_RS27970	5499258..5499500	-	243	WP_001086390.1	hypothetical protein	-
HZT35_RS27975	5499719..5499937	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
HZT35_RS27980	5500542..5500832	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
HZT35_RS27985	5500833..5501144	-	312	WP_000897305.1	hypothetical protein	Toxin
HZT35_RS27990	5501373..5502281	+	909	WP_001162704.1	alpha/beta hydrolase	-
HZT35_RS27995	5502345..5503286	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
HZT35_RS28000	5503331..5503768	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HZT35_RS28005	5503765..5504637	-	873	WP_000920762.1	virulence factor BrkB family protein	-
HZT35_RS28010	5504631..5505230	-	600	WP_001315111.1	glucose-1-phosphatase	-
HZT35_RS28015	5505329..5506114	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	5498049..5499029	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T167167 WP_000897305.1 NZ_CP058682:c5501144-5500833 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T167167 NZ_CP074712:c6692-6405 [Acinetobacter baumannii]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTCGAAACTGCAAG
CCTTGTTTTTGAAGATCCATTACGGATCTCAATCCAAGACAGACATACCAATGGTGAAGAACGTTGGCAAACCATTGGAC
GAGTTAAAGGCGTACTAATGTTATTGGTGGCTCACACCATTTTTGATGAAGATGACTGTGAAATCATCCGAATCATTAGT
GCAAGGCAAGTCACTAAAGCGGAGCGAAACAAATATGAGCATGGTTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT167167 WP_000356397.1 NZ_CP058682:c5500832-5500542 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT167167 NZ_CP074712:c6418-6104 [Acinetobacter baumannii]
ATGAGCATGGTTAGATACTCACGCAAAGAGCTAAATGAGAAATTTGGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACGGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACAGACTGGAGCAATGCTGTACGCC
AAAATCAATTTTACCGCCCAGTAAAGCAACAAACTTCAATTCGGTTAGATGCTGATGTACTTGCTTGGTTCAAAGCACAA
GGCAAAGGTTATCAAACACGTATGAATGAAATTTTACGAGATGCTATGCTAAAAGAATTAAAAAATCATCAATAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7