Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4569634..4570169 Replicon chromosome
Accession NZ_CP058682
Organism Escherichia coli strain MBT-5

Toxin (Protein)


Gene name yafQ Uniprot ID A0A0F3UZU8
Locus tag HZT35_RS23620 Protein ID WP_032183258.1
Coordinates 4569882..4570169 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag HZT35_RS23615 Protein ID WP_000729704.1
Coordinates 4569634..4569894 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT35_RS23590 4564670..4565809 + 1140 WP_000785656.1 flagellar type III secretion system protein FlhB -
HZT35_RS23595 4565793..4567886 + 2094 WP_001140806.1 flagellar biosynthesis protein FlhA -
HZT35_RS23600 4567991..4568269 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
HZT35_RS23605 4568262..4568618 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
HZT35_RS23610 4568675..4569448 - 774 WP_000543897.1 C40 family peptidase -
HZT35_RS23615 4569634..4569894 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
HZT35_RS23620 4569882..4570169 + 288 WP_032183258.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
HZT35_RS23625 4570325..4571065 + 741 WP_001225679.1 murein L,D-transpeptidase -
HZT35_RS23630 4571036..4571803 - 768 WP_000333380.1 class II glutamine amidotransferase -
HZT35_RS23635 4572009..4572587 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(7-94)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11166.96 Da        Isoelectric Point: 10.2267

>T167163 WP_032183258.1 NZ_CP058682:4569882-4570169 [Escherichia coli]
IRNLNQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDK
LLRFERTGTHAALFG

Download         Length: 288 bp

>T167163 NZ_CP074711:423-779 [Acinetobacter baumannii]
ATGTGGACAGTCATTACAACGGATCTGTTTAACGAATGGCTTGTACAGCAAGATGAATCTACACAAGAGAAAGTACTCGC
AGCGTTGGTCGTTCTACAGCAACAGGGACCAAGTTTAGGTCGTCCTTTAGTAGATACCGTCTATGACTCCAAATTTACCA
ACATGAAAGAGCTACGTGTTCAGCATCGAGGTAAACCACTGAGAGCCTTTTTCGCATTTGATCCTTTACGACAAGCTATT
GTGCTGTGTATCGGTGATAAAGGCGGTAAAAAGCGTTTTTATAAAGAGATGTTAGATATTGCCGATCAGCAATACGAACT
TCATCTTTCAACTTTAGGAGATCAATCTAATGGCTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT167163 WP_000729704.1 NZ_CP058682:4569634-4569894 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT167163 NZ_CP074711:772-1074 [Acinetobacter baumannii]
ATGGCTAAGACCCTACAAGAATTATTGGCTAGTCGCTCTCCAGAGAGTCAAGCTCGTATTCAAAAAATGGCTGATGAATT
ACTGTTGGAAACTCAGCTTCACCTTATTCGTGAAGAGTTAGAGATTTCTCAAAAAGAACTGGCTGCAACCCTTGGAATCA
AACAGCCGTCATTGTCAGCAATCGAAAATCGTGGTCATGATCTTAAAATTTCAACGATGAAAAAGTATGTTGAAGCAATG
GGCGGAAAACTTCGTATTGATGTAGAGCTTCCAACAGGAAAACACATAGGATTTAATGTCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0F3UZU8

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References