Detailed information of TA system TA167152

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 2478494..2478865
• Replicon: chromosome
• Accession: NZ_CP058682
• Organism: Escherichia coli strain MBT-5

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A891STD1
• Locus tag: HZT35_RS12510
• Protein ID: WP_042853000.1
• Coordinates: 2478671..2478865 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2478494..2478672 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZT35_RS12470	2473936..2474592	-	657	WP_000011658.1	carbon-nitrogen hydrolase family protein	-
HZT35_RS12475	2474694..2474924	-	231	WP_000916763.1	DNA polymerase III subunit theta	-
HZT35_RS12480	2475063..2475437	+	375	WP_000204699.1	CopC domain-containing protein YobA	-
HZT35_RS12485	2475441..2476313	+	873	WP_000879280.1	copper homeostasis membrane protein CopD	-
HZT35_RS12490	2476326..2476667	+	342	WP_000976492.1	YebY family protein	-
HZT35_RS12495	2477060..2478136	-	1077	WP_001189091.1	phage integrase Arm DNA-binding domain-containing protein	-
HZT35_RS12500	2478102..2478383	-	282	WP_001443927.1	excisionase	-
HZT35_RS12505	2478490..2478678	-	189	WP_001356607.1	DUF1187 family protein	-
-	2478494..2478672	+	179	NuclAT_1	-	Antitoxin
-	2478494..2478672	+	179	NuclAT_1	-	Antitoxin
-	2478494..2478672	+	179	NuclAT_1	-	Antitoxin
-	2478494..2478672	+	179	NuclAT_1	-	Antitoxin
HZT35_RS12510	2478671..2478865	-	195	WP_042853000.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
HZT35_RS12515	2478922..2479731	-	810	WP_000166317.1	recombination protein RecT	-
HZT35_RS12520	2479724..2482375	-	2652	WP_000105101.1	exodeoxyribonuclease VIII	-
HZT35_RS12525	2482474..2482749	-	276	WP_001307773.1	hypothetical protein	-
HZT35_RS12530	2482823..2482999	-	177	WP_000245534.1	hypothetical protein	-
HZT35_RS12535	2482993..2483214	-	222	WP_000560218.1	killing protein KilR	-
HZT35_RS12540	2483635..2483787	-	153	WP_001427316.1	DUF1391 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2476326..2544736	68410

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7019.87 Da
• Isoelectric Point: 8.6419

>T167152 WP_042853000.1 NZ_CP058682:c2478865-2478671 [Escherichia coli]
MRYDDVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T167152 NZ_CP074703:c5488-5213 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGTCGCGGTTATTTGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAAATTAATGGCCAGACTATTTAT
GTATTGCGTCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Nucleotide

• Length: 179 bp

>AT167152 NZ_CP058682:2478494-2478672 [Escherichia coli]
GAGGACAGAAGTTTCTCGCAATTAAAATTTATCAGCTTTACTTTCTGCTCTCTGGACACGCCTGCTTCTTTTTTCCCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAATTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCTTTTT
CAATAGTGGCAGTTATTTT

Structures

Antitoxin