Detailed information of TA system TA167138

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1108119..1108846
• Replicon: chromosome
• Accession: NZ_CP058682
• Organism: Escherichia coli strain MBT-5

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I2UEW4
• Locus tag: HZT35_RS05355
• Protein ID: WP_000547559.1
• Coordinates: 1108119..1108430 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HZT35_RS05360
• Protein ID: WP_000126294.1
• Coordinates: 1108427..1108846 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZT35_RS05325	1103261..1104970	+	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-
HZT35_RS05330	1104980..1105522	+	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
HZT35_RS05335	1105522..1106289	+	768	WP_182111023.1	formate hydrogenlyase subunit HycG	-
HZT35_RS05340	1106286..1106696	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
HZT35_RS05345	1106689..1107159	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
HZT35_RS05350	1107202..1107945	+	744	WP_001341829.1	hypothetical protein	-
HZT35_RS05355	1108119..1108430	+	312	WP_000547559.1	type II toxin-antitoxin system HigB family toxin	Toxin
HZT35_RS05360	1108427..1108846	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
HZT35_RS05365	1108960..1110384	-	1425	WP_000110313.1	6-phospho-beta-glucosidase AscB	-
HZT35_RS05370	1110393..1111850	-	1458	WP_024017722.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
HZT35_RS05375	1112110..1113120	+	1011	WP_001377591.1	DNA-binding transcriptional regulator AscG	-
HZT35_RS05380	1113269..1113796	+	528	WP_001078781.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (24-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12450.11 Da
• Isoelectric Point: 9.7249

>T167138 WP_000547559.1 NZ_CP058682:1108119-1108430 [Escherichia coli]
MHIISKAPFEESARKYPNDALAHQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T167138 NZ_CP074701:c2159081-2158674 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATTCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTACAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT167138 WP_000126294.1 NZ_CP058682:1108427-1108846 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT167138 NZ_CP074701:c2159328-2159062 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A0F3V163