Detailed information of TA system TA167100

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 1617620..1618246
• Replicon: chromosome
• Accession: NZ_CP058668
• Organism: Treponema sp. B152

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q73NN6
• Locus tag: HYB91_RS07525
• Protein ID: WP_002677634.1
• Coordinates: 1617620..1617928 (+)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HYB91_RS07530
• Protein ID: WP_253682324.1
• Coordinates: 1617938..1618246 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HYB91_RS07500 (HYB91_07465)	1613572..1613910	+	339	WP_010696721.1	anti-sigma factor antagonist	-
HYB91_RS07505 (HYB91_07470)	1613927..1614469	+	543	WP_253719454.1	ATP-binding protein	-
HYB91_RS07510 (HYB91_07475)	1614477..1615304	-	828	WP_253719455.1	hypothetical protein	-
HYB91_RS07515 (HYB91_07480)	1615285..1615905	-	621	WP_253719456.1	hypothetical protein	-
HYB91_RS07520 (HYB91_07485)	1616022..1617401	+	1380	WP_253719457.1	tyrosine phenol-lyase	-
HYB91_RS07525 (HYB91_07490)	1617620..1617928	+	309	WP_002677634.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HYB91_RS07530 (HYB91_07495)	1617938..1618246	+	309	WP_253682324.1	NadS family protein	Antitoxin
HYB91_RS07535 (HYB91_07500)	1618380..1619051	+	672	WP_253719458.1	TetR/AcrR family transcriptional regulator	-
HYB91_RS07540 (HYB91_07505)	1619140..1619556	+	417	WP_253719459.1	SRPBCC family protein	-
HYB91_RS07545 (HYB91_07510)	1619699..1620328	-	630	WP_253719460.1	adenylate kinase	-
HYB91_RS07550 (HYB91_07515)	1620384..1621613	-	1230	WP_010696711.1	cation:dicarboxylase symporter family transporter	-
HYB91_RS07555 (HYB91_07520)	1621652..1622971	-	1320	WP_002670665.1	leucine-rich repeat domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (43-86)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11736.96 Da
• Isoelectric Point: 10.6864

>T167100 WP_002677634.1 NZ_CP058668:1617620-1617928 [Treponema sp. B152]
MRIIETPVFTKQINRLLDEDTYKQFKEYLVCNPLKGKLIKGGGGIRKIRWSKKNTGKSGGIRIIYFIKTETKIYLLFAYS
KSDAESITKKQINMLASFIKGL

Nucleotide

• Length: 309 bp

>T167100 NZ_CP074680:1856417-1856668 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11549.43 Da
• Isoelectric Point: 9.6884

>AT167100 WP_253682324.1 NZ_CP058668:1617938-1618246 [Treponema sp. B152]
MFKRKKMSDEMFAELLQSVKEAVLIEKGEIPPSRVFEIEPLDIAKIRSKTNKTQEEFASMLNISIGTLRNWEQGRRKPDG
AALSLLKIVSANPQYVESVLQG

Nucleotide

• Length: 309 bp

>AT167100 NZ_CP074680:1856665-1856919 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: Q73NN6