Detailed information of TA system TA167089

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 106631..106864
• Replicon: plasmid pCTXM15_005008
• Accession: NZ_CP058663
• Organism: Escherichia coli strain 005008

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: HZR83_RS24270
• Protein ID: WP_001312861.1
• Coordinates: 106631..106789 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	106833..106864 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZR83_RS24245	102005..102694	-	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-
HZR83_RS24250	102881..103264	-	384	WP_001151524.1	relaxosome protein TraM	-
HZR83_RS24255	103585..104187	+	603	WP_013362798.1	transglycosylase SLT domain-containing protein	-
HZR83_RS24260	104484..105305	-	822	WP_001234426.1	DUF945 domain-containing protein	-
HZR83_RS24265	105423..105710	-	288	WP_000107535.1	hypothetical protein	-
HZR83_RS24270	106631..106789	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	106833..106864	-	32	NuclAT_1	-	Antitoxin
-	106833..106864	-	32	NuclAT_1	-	Antitoxin
-	106833..106864	-	32	NuclAT_1	-	Antitoxin
-	106833..106864	-	32	NuclAT_1	-	Antitoxin
-	108306..108503	-	198	NuclAT_0	-	-
-	108306..108503	-	198	NuclAT_0	-	-
-	108306..108503	-	198	NuclAT_0	-	-
-	108306..108503	-	198	NuclAT_0	-	-
HZR83_RS24280	108315..108503	+	189	WP_001299721.1	hypothetical protein	-
HZR83_RS24285	108515..109234	-	720	WP_001276238.1	plasmid SOS inhibition protein A	-
HZR83_RS24290	109231..109665	-	435	WP_000845937.1	conjugation system SOS inhibitor PsiB	-
HZR83_RS24295	109720..109917	-	198	Protein_131	hypothetical protein	-
HZR83_RS24300	109945..110178	-	234	WP_000005990.1	DUF905 family protein	-
HZR83_RS24305	110246..110785	-	540	WP_000290840.1	single-stranded DNA-binding protein	-
HZR83_RS24310	110811..111017	-	207	WP_000275856.1	hypothetical protein	-
HZR83_RS24315	111427..111639	-	213	WP_001348622.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(B) / mph(A) / sul1 / qacE / aadA5 / blaCTX-M-15 / aac(3)-IIa / blaOXA-1 / aac(6')-Ib-cr / sitABCD	iutA / iucD / iucC / iucB / iucA	1..149236	149236

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T167089 WP_001312861.1 NZ_CP058663:c106789-106631 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T167089 NZ_CP074677:c4712961-4712044 [Klebsiella pneumoniae]
ATGGATATACAGGCGCCTTATTATCGGCAGGTTGCTCTGCTAATGCAGGTTTTACCCTATGTTGCTGTCGAACGGGAATT
TGCGCTGAAAGGTGGCACTGCAATCAATCTCTTTATCCGCGATTTCCCTCGATTATCGGTGGATATCGACCTCGCCTGGG
TGCCCCTGGAAAGCAGAGCCATCGCTTTACCTCATATACGGGATGCGCTTGCACGTATTGCCGCCAATCTGCAACAACAG
GCAGGTATGAGCGCTGTATTACAAGCTAATCGTTCAGATGAGATGCGTGTCATTGTTACTACCGATAGCGCACAAATAAA
GATAGAGGTTTCACCGGTCGCCCGGGGAACATTATATCCACCACAGGAGCGAGAAGTGGTGGGGGAGGTAGAGGACGTTT
TTGGCTATGCCAGCCTTCCAGTGGTTTCGTTGCCTGACCTCTACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCAT
CCCAGAGATTTTTATGACGTGAAATTACTTCTTGATGCGCAGGAGCTTGACCGTCCCATTTTCAATGGGTTTATCGCGTA
TTTACTGAGCCATAATCGGCCTCTGGCTGAAGTGCTGAGTCCTCGCTGGAAAGATATTGCTGAACCTTTTTACCGTGAAT
TCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGACAGCAGTCCCCAACAGGATGATTGCTGCGTTAAAATCC
TGTTTCACACAACAGGATGTCTATTTTTTACTGTCATTTAAACGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAATGCG
TATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATATTCATCAGATGCCAGCGATAAAACGCGCTGAGTCATTAG
ATAAACTGGAAAAGGTGCTGGCCGAGTGGCGCAGTTAG

Antitoxin

Nucleotide

• Length: 32 bp

>AT167089 NZ_CP058663:c106864-106833 [Escherichia coli]
CACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin