Detailed information of TA system TA167077

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4370996..4371723
• Replicon: chromosome
• Accession: NZ_CP058661
• Organism: Escherichia coli strain 005008

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A829L2T9
• Locus tag: HZR83_RS20935
• Protein ID: WP_001521139.1
• Coordinates: 4371412..4371723 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HZR83_RS20930
• Protein ID: WP_000126294.1
• Coordinates: 4370996..4371415 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZR83_RS20910	4366081..4366608	-	528	WP_001078777.1	electron transport protein HydN	-
HZR83_RS20915	4366757..4367767	-	1011	WP_015912547.1	DNA-binding transcriptional regulator AscG	-
HZR83_RS20920	4368027..4369484	+	1458	WP_020234027.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
HZR83_RS20925	4369493..4370917	+	1425	WP_020234028.1	6-phospho-beta-glucosidase AscB	-
HZR83_RS20930	4370996..4371415	-	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
HZR83_RS20935	4371412..4371723	-	312	WP_001521139.1	type II toxin-antitoxin system HigB family toxin	Toxin
HZR83_RS20940	4371890..4372360	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
HZR83_RS20945	4372353..4372763	-	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
HZR83_RS20950	4372760..4373527	-	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
HZR83_RS20955	4373527..4374069	-	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
HZR83_RS20960	4374079..4375788	-	1710	WP_001521140.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12493.23 Da
• Isoelectric Point: 9.4783

>T167077 WP_001521139.1 NZ_CP058661:c4371723-4371412 [Escherichia coli]
MHIISKAPFEECARKYPNDALALYSLYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T167077 NZ_CP074677:327826-328194 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT167077 WP_000126294.1 NZ_CP058661:c4371415-4370996 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT167077 NZ_CP074677:327608-327829 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: A0A829L2T9