Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 3606369..3606871 Replicon chromosome
Accession NZ_CP058661
Organism Escherichia coli strain 005008

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag HZR83_RS17390 Protein ID WP_000767829.1
Coordinates 3606369..3606623 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag HZR83_RS17395 Protein ID WP_001259255.1
Coordinates 3606620..3606871 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZR83_RS17360 3601384..3601611 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
HZR83_RS17365 3601625..3602683 - 1059 WP_000492333.1 transport protein YeeE -
HZR83_RS17370 3602862..3604220 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
HZR83_RS17375 3604210..3604272 - 63 WP_010723108.1 membrane protein YoeI -
HZR83_RS17380 3604487..3605416 - 930 WP_000803351.1 LysR family transcriptional regulator -
HZR83_RS17385 3605462..3606286 - 825 WP_000754749.1 SDR family oxidoreductase -
HZR83_RS17390 3606369..3606623 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
HZR83_RS17395 3606620..3606871 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
HZR83_RS17400 3607155..3607205 + 51 WP_001364200.1 his operon leader peptide -
HZR83_RS17405 3607351..3608250 + 900 WP_032329340.1 ATP phosphoribosyltransferase -
HZR83_RS17410 3608256..3609560 + 1305 WP_001446927.1 histidinol dehydrogenase -
HZR83_RS17415 3609557..3610627 + 1071 WP_000108945.1 histidinol-phosphate transaminase -
HZR83_RS17420 3610627..3611694 + 1068 WP_046464156.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T167076 WP_000767829.1 NZ_CP058661:c3606623-3606369 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T167076 NZ_CP074676:1020958-1021257 [Pseudomonas qingdaonensis]
ATGAGCGTTACATTGATTCCATTCGACATGGCGGCACTGCTTGATAGTGATGAAGCCATCAGCGAGTACTTGTCTCAGGT
GCTGGCAGAGGGCGACAGCGACGAGCTTTTACGTGCAATAGGGTACGTGGCCAAAGCGCGCGGAATGTCGCTGGTTGCCA
AAGAGACTGGATTGGGAAGAGAGAGCCTTTACAAGGCCTTCGCGCCAGGCGCCAAGCCTCGTTTCGATACGGTGCTGAAG
GTGATGCATGCGCTGGGAATCGAACTCTGTGCGCGCTCGCATCGAGGGGCTTCGGTGTAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT167076 WP_001259255.1 NZ_CP058661:c3606871-3606620 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT167076 NZ_CP074676:1020662-1020955 [Pseudomonas qingdaonensis]
GTGATCTATTCCATAGAACAGACTTTGGCATTCAGCGGCTGGCTTCTGGCGCTACGCGATGTGCGAGCGAGGATCGCCAT
TGGTCGACGCATCGAACGTGCGCTTGCGGGCAACTTGGGGGATACGAAGGCGGTTGGTGATGGTGTTTCAGAGATGCGCA
TCGATTCCGGCCCAGGCTATCGGGTGTACTTCACCGTGCGTAGCGGCGTAGCAATCTTGTTGCTGACAGGCGGGGACAAG
TCTTCTCAAGCAGCAGATATCCGGATGGCCAAGCGGTTGGCCAAAGAGGTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References