Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 1796360..1797054 Replicon chromosome
Accession NZ_CP058661
Organism Escherichia coli strain 005008

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag HZR83_RS08560 Protein ID WP_001263489.1
Coordinates 1796656..1797054 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag HZR83_RS08555 Protein ID WP_000554758.1
Coordinates 1796360..1796653 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZR83_RS08535 1791992..1792489 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
HZR83_RS08540 1792713..1794452 - 1740 WP_001334802.1 flagellar biosynthesis protein FlhA -
HZR83_RS08545 1794397..1795182 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
HZR83_RS08550 1795253..1796308 + 1056 WP_001226164.1 DNA polymerase IV -
HZR83_RS08555 1796360..1796653 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
HZR83_RS08560 1796656..1797054 + 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
HZR83_RS08565 1797064..1797516 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
HZR83_RS08570 1797834..1798040 + 207 Protein_1660 RtcB family protein -
HZR83_RS08575 1798036..1798557 + 522 Protein_1661 peptide chain release factor H -
HZR83_RS08580 1798614..1800071 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
HZR83_RS08585 1800332..1800790 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- 1801386..1801466 + 81 NuclAT_12 - -
- 1801386..1801466 + 81 NuclAT_12 - -
- 1801386..1801466 + 81 NuclAT_12 - -
- 1801386..1801466 + 81 NuclAT_12 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T167063 WP_001263489.1 NZ_CP058661:1796656-1797054 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T167063 NZ_CP074663:3515225-3515443 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT167063 WP_000554758.1 NZ_CP058661:1796360-1796653 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT167063 NZ_CP074663:3514823-3515197 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCCAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References