Detailed information of TA system TA167049

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 81977..82231
• Replicon: plasmid p1_035125
• Accession: NZ_CP058658
• Organism: Escherichia coli strain WCHEC035125

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: HZR25_RS23255
• Protein ID: WP_015387399.1
• Coordinates: 81977..82183 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	82170..82231 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZR25_RS23225	77224..78135	-	912	WP_000440183.1	carbamate kinase	-
HZR25_RS23230	78146..79366	-	1221	WP_000410951.1	arginine deiminase	-
HZR25_RS23235	80259..80741	+	483	Protein_85	VENN motif pre-toxin domain-containing protein	-
HZR25_RS23240	80687..81133	-	447	Protein_86	incFII family plasmid replication initiator RepA	-
HZR25_RS23245	81126..81200	-	75	WP_032336874.1	RepA leader peptide Tap	-
HZR25_RS23250	81436..81693	-	258	WP_000083833.1	replication regulatory protein RepA	-
HZR25_RS23255	81977..82183	-	207	WP_015387399.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	82170..82231	+	62	NuclAT_0	-	Antitoxin
-	82170..82231	+	62	NuclAT_0	-	Antitoxin
-	82170..82231	+	62	NuclAT_0	-	Antitoxin
-	82170..82231	+	62	NuclAT_0	-	Antitoxin
HZR25_RS23260	82370..82552	-	183	WP_000968309.1	hypothetical protein	-
HZR25_RS23265	82653..83269	+	617	Protein_91	IS1 family transposase	-
HZR25_RS23270	83307..84878	-	1572	WP_023149734.1	IS66 family transposase	-
HZR25_RS23275	84898..85245	-	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
HZR25_RS23280	85245..85922	-	678	WP_001339397.1	IS66 family insertion sequence hypothetical protein	-
HZR25_RS23285	85977..86066	+	90	Protein_95	IS1 family transposase	-
HZR25_RS23290	86367..86579	-	213	WP_005012601.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	dfrA14 / qnrS1 / tet(A) / sitABCD	iutA / iucD / iucC / iucB / iucA	1..122305	122305

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7720.23 Da
• Isoelectric Point: 9.3127

>T167049 WP_015387399.1 NZ_CP058658:c82183-81977 [Escherichia coli]
MKYLNTTDCSLFLAGRSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T167049 NZ_CP074661:c4020560-4020252 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGCAATACATGGTTTATCGCAATAAAGGAAACAGCAAGGCTTATCCTTATTTACTTGATGTGCAAAGCGATATTATTGA
TGAACTGCATACTCGCATGGTTATCCCATTGTTTCCTGTGAGCAGATTGGTAAATAACCCAGTTAAGCGCCTCACGCCCA
CTCTGAATGTGGAAGGTAATGACTATCTGGTGATGACTCACGAAATGGCGAGTATCAGGCTTTCGCAGATTGGTGATGAG
GTGATGGACGTTCGTTCCCACAGGCAAACCATCAAAAATGCTCTCGATTTTATCTTTGACGGGTTCTGA

Antitoxin

Nucleotide

• Length: 62 bp

>AT167049 NZ_CP058658:82170-82231 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin