Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 643891..644690 Replicon chromosome
Accession NZ_CP058656
Organism Escherichia coli strain WCHEC035125

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag HZR25_RS03125 Protein ID WP_000347251.1
Coordinates 643891..644355 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag HZR25_RS03130 Protein ID WP_001307405.1
Coordinates 644355..644690 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZR25_RS03095 638892..639326 - 435 WP_000948824.1 PTS sugar transporter subunit IIA -
HZR25_RS03100 639344..640222 - 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
HZR25_RS03105 640212..640991 - 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
HZR25_RS03110 641002..641475 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
HZR25_RS03115 641498..642778 - 1281 WP_000681903.1 tagatose-bisphosphate aldolase subunit KbaZ -
HZR25_RS03120 643027..643836 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
HZR25_RS03125 643891..644355 - 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
HZR25_RS03130 644355..644690 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
HZR25_RS03135 644839..646410 - 1572 WP_001273761.1 galactarate dehydratase -
HZR25_RS03140 646785..648119 + 1335 WP_000599652.1 galactarate/glucarate/glycerate transporter GarP -
HZR25_RS03145 648135..648905 + 771 WP_001058214.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T167026 WP_000347251.1 NZ_CP058656:c644355-643891 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T167026 NZ_CP074606:307415-307783 [Salmonella enterica subsp. enterica serovar Newport str.]
ATGACCCTACAACTTATCTCAGCGGAAGAGATAATTCAGTTTCACGACAGGCTTCTCCGCGTTACGCCTGGTGTAACAGG
CATGCCTGATCCTGGCCGCGCGGAAGCGCTAATGTACCGGGTACTCAATCAAATCGAATATGAAGGGGTGACCGACGTGT
GGCTGCTGGCGGCAATGCATTTGCTCGCTATATCCCGTGGGCATATCTTCAATGATGGTAACAAACGTACCGCCTTATTT
ATTACGCTGCTGTTTTTAAAGCGTAACGGGATCTCACTCGCTGCGAATCCGGATTTTGTCGATATGACAGTCGATGCGGC
GGCAGGGCGGCTTACGCTGGAGCAAATTGTCGTTCGCTTACGCGCCTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT167026 WP_001307405.1 NZ_CP058656:c644690-644355 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT167026 NZ_CP074606:307197-307418 [Salmonella enterica subsp. enterica serovar Newport str.]
ATGCGTACGGTTAACTATAGCGAAGCGCGACAAAATCTGGCCGAAGTCCTGGAAAGCGCGGTGACGGCGGGCCCTGTCAC
CATCACGCGTCGTGGGCATAAGTCCGCAGTGATCATCAGCGCCGAGGAGTTTGAGCGTTATCAGACGGCGCGAATGGATG
ATGAGTTTGCTGCCATTATGGCGGTTCATGGCAATGAGCTCAGGGAGCTGGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References