Detailed information of TA system TA167023

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 31280..31991
• Replicon: plasmid p2_035123
• Accession: NZ_CP058654
• Organism: Escherichia coli strain WCHEC035123

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I2UJZ0
• Locus tag: HZR24_RS23825
• Protein ID: WP_000162415.1
• Coordinates: 31280..31582 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HZR24_RS23830
• Protein ID: WP_000806445.1
• Coordinates: 31653..31991 (+)
• Length: 113 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZR24_RS23795	26299..26979	-	681	WP_000812238.1	hypothetical protein	-
HZR24_RS23800	26963..27868	-	906	WP_112031934.1	recombination-associated protein RdgC	-
HZR24_RS23805	27928..28581	-	654	WP_087503501.1	baseplate protein	-
HZR24_RS23810	28578..29558	-	981	WP_000046500.1	hypothetical protein	-
HZR24_RS23815	29562..30329	-	768	WP_000203293.1	hypothetical protein	-
HZR24_RS23820	30326..31117	-	792	WP_112031933.1	phage tail protein	-
HZR24_RS23825	31280..31582	+	303	WP_000162415.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HZR24_RS23830	31653..31991	+	339	WP_000806445.1	HigA family addiction module antidote protein	Antitoxin
HZR24_RS23835	32270..32998	+	729	WP_109157909.1	hypothetical protein	-
HZR24_RS23840	33032..34225	-	1194	WP_074470350.1	phage tail protein	-
HZR24_RS23845	34218..34547	-	330	WP_112031932.1	baseplate protein	-
HZR24_RS23850	34876..35529	-	654	WP_001557715.1	hypothetical protein	-
HZR24_RS23855	35813..36421	+	609	WP_000776434.1	hypothetical protein	-
HZR24_RS23860	36607..36834	+	228	WP_001396850.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..89623	89623

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (6-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-69)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11818.56 Da
• Isoelectric Point: 9.8739

>T167023 WP_000162415.1 NZ_CP058654:31280-31582 [Escherichia coli]
MTKKINIKDFRDAWLDDFFEFSTPHRKIPPDIHMTLSRKLDIINAATTCKDLRSPPGNRYEELSGKLNGYSSVRVNKQYR
LIFKWVNGKAEDLYLDPHKY

Nucleotide

• Length: 303 bp

>T167023 NZ_CP074592:c3992291-3991803 [Salmonella bongori serovar 40:z35:-]
ATGATCTCCGCCCCTGAACCGCTTCATGCCGGGCATATTCTTACCCCCTTCTGCTGCGGTGTGGATTCAATGGATAACTG
GCTAAAACAGCGGGCGATGAAAAATCAGGTAACTGGCGCATCCCGTACCTTTGTGTGTTGCGGCAGCGATTCTGACGTAC
TGGCGTATTATTCGCTGGCGTCCAGCGCGGTAACGACAAACACCGCCCCCGGTCGCTTTCGTCGCAATATGCCCGATCCG
ATCCCGGTTGTGGTGTTGGGCCGTCTGGCGGTGGATCGGTCGCTACATGGACAGGGCGTAGGTCGGGCGCTGGTGAGGGA
TGCCGGGCTGCGGGTGATTCAGGTGGCGGAAACTATCGGTATTCGCGGGATGCTGGTCCATGCCCTGTCGGATGAAGCGC
GGGAGTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGGGGGATTTGGTT
CATGCCTGA

Antitoxin

Protein

• Length: 113 a.a.
• Molecular weight: 13049.98 Da
• Isoelectric Point: 6.4754

>AT167023 WP_000806445.1 NZ_CP058654:31653-31991 [Escherichia coli]
MKQATRKPTTVGDILLYEYLEPLELKINELAEILHVHRNTVSALVNNNRKLTMDMAYRLAKAFDTSVDFWINLQTAVDLW
EVENDMRVQEELSRINTAEKFISQRNLNKKAA

Nucleotide

• Length: 339 bp

>AT167023 NZ_CP074592:c3992581-3992288 [Salmonella bongori serovar 40:z35:-]
ATGCCAGCCGCAAACAGTATGGCGATGAAACGCGAAACCCTGAATCTGCGCATCAAACCCGCTGAGCGTGATCTTATCGA
TCGGGCAGCGAAAGCCAGAGGGAAAAACCGCACTGACTTTGTGCTGGAAGCCGCCCGCGCTGCCGCAGAGGAAGCGCTGA
TCGAGCAACGCATCATTATGGCCGATCCAGAGGCTTATCAGGCATTTCTGACCCGTCTGGATCAGGCTCCTGCGCCAAAC
GATGCGTTGCGTAAAACCATGCAGACTCCTGCACCGTGGGAGCAGGAAAAATGA

Structures

Toxin

• AlphaFold DB: A0A631VBQ3