Detailed information of TA system TA166998

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1108878..1109605
• Replicon: chromosome
• Accession: NZ_CP058651
• Organism: Escherichia coli strain WCHEC035123

Toxin (Protein)

• Gene name: higB
• Uniprot ID: J7Q991
• Locus tag: HZR24_RS05390
• Protein ID: WP_000547564.1
• Coordinates: 1108878..1109189 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HZR24_RS05395
• Protein ID: WP_000126294.1
• Coordinates: 1109186..1109605 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZR24_RS05360	1104020..1105729	+	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-
HZR24_RS05365	1105739..1106281	+	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
HZR24_RS05370	1106281..1107048	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
HZR24_RS05375	1107045..1107455	+	411	WP_001291921.1	formate hydrogenlyase assembly protein HycH	-
HZR24_RS05380	1107448..1107918	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
HZR24_RS05385	1107943..1108704	+	762	WP_001026446.1	hypothetical protein	-
HZR24_RS05390	1108878..1109189	+	312	WP_000547564.1	type II toxin-antitoxin system HigB family toxin	Toxin
HZR24_RS05395	1109186..1109605	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
HZR24_RS05400	1109719..1111143	-	1425	WP_000110363.1	6-phospho-beta-glucosidase AscB	-
HZR24_RS05405	1111152..1112609	-	1458	WP_001107861.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
HZR24_RS05410	1112869..1113879	+	1011	WP_001392554.1	DNA-binding transcriptional regulator AscG	-
HZR24_RS05415	1114028..1114555	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12426.13 Da
• Isoelectric Point: 9.7248

>T166998 WP_000547564.1 NZ_CP058651:1108878-1109189 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T166998 NZ_CP074580:3709487-3709897 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT166998 WP_000126294.1 NZ_CP058651:1109186-1109605 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT166998 NZ_CP074580:3709240-3709506 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A0A0FJG5