Detailed information of TA system TA166990

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 6337631..6338238
• Replicon: chromosome
• Accession: NZ_CP058644
• Organism: Pseudomonas yamanorum strain GZD14026

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: HZF02_RS29385
• Protein ID: WP_179448331.1
• Coordinates: 6337924..6338238 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HZF02_RS29380
• Protein ID: WP_124369841.1
• Coordinates: 6337631..6337924 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZF02_RS29350	6332934..6333356	+	423	WP_179451255.1	hypothetical protein	-
HZF02_RS29355	6333346..6334275	+	930	WP_179448327.1	hypothetical protein	-
HZF02_RS29360	6334272..6334817	+	546	WP_179448328.1	DNA-binding protein	-
HZF02_RS29365	6334814..6335518	+	705	WP_179448329.1	hypothetical protein	-
HZF02_RS29370	6335491..6336462	+	972	WP_179448330.1	hypothetical protein	-
HZF02_RS29375	6336470..6337489	-	1020	WP_179451257.1	acyltransferase	-
HZF02_RS29380	6337631..6337924	-	294	WP_124369841.1	helix-turn-helix domain-containing protein	Antitoxin
HZF02_RS29385	6337924..6338238	-	315	WP_179448331.1	hypothetical protein	Toxin
HZF02_RS29390	6338328..6338675	-	348	WP_179448332.1	hypothetical protein	-
HZF02_RS29395	6339181..6340347	-	1167	WP_179448333.1	hypothetical protein	-
HZF02_RS29400	6340344..6340925	-	582	WP_179448334.1	LemA family protein	-
HZF02_RS29405	6341054..6341521	+	468	WP_179448335.1	hypothetical protein	-
HZF02_RS29410	6341659..6342309	-	651	Protein_5793	AarF/ABC1/UbiB kinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	6285909..6347974	62065
	-	flank	IS/Tn	-	-	6342365..6343393	1028

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-72)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11777.46 Da
• Isoelectric Point: 8.3905

>T166990 WP_179448331.1 NZ_CP058644:c6338238-6337924 [Pseudomonas yamanorum]
MIFIESPIFTEDLGELLSDDSYREFQEYLAENPTAGDVIQGTNGLRKVRWSANGKGKSGGVRVIYFHLSAAYQIRLILIY
RKGIKDDLSPAEKKVLAELNRGWK

Nucleotide

• Length: 315 bp

>T166990 NZ_CP074580:c1488630-1488313 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGTCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAAATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11001.59 Da
• Isoelectric Point: 10.1711

>AT166990 WP_124369841.1 NZ_CP058644:c6337924-6337631 [Pseudomonas yamanorum]
MEKNLFNRLVESMTQMDEIDRGERPPSREFHVDAVKVKMIRKATGLSQARFAKAIDVAVGTLQNWEQGRREPEGPARALL
RAIHNDPGHVLAALEQR

Nucleotide

• Length: 294 bp

>AT166990 NZ_CP074580:c1488320-1488033 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA