Detailed information of TA system TA166966

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 1616104..1616730
• Replicon: chromosome
• Accession: NZ_CP058624
• Organism: Treponema denticola strain OMZ 854

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q73NN6
• Locus tag: HO345_RS07630
• Protein ID: WP_002677634.1
• Coordinates: 1616104..1616412 (+)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HO345_RS07635
• Protein ID: WP_253682324.1
• Coordinates: 1616422..1616730 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HO345_RS07600 (HO345_07595)	1612055..1612393	+	339	WP_010696721.1	anti-sigma factor antagonist	-
HO345_RS07605 (HO345_07600)	1612410..1612952	+	543	WP_253682318.1	ATP-binding protein	-
HO345_RS07610 (HO345_07605)	1612967..1613788	-	822	WP_253682319.1	hypothetical protein	-
HO345_RS07615 (HO345_07610)	1613769..1614389	-	621	WP_253682320.1	hypothetical protein	-
HO345_RS07620 (HO345_07615)	1614506..1615885	+	1380	WP_253682321.1	tyrosine phenol-lyase	-
HO345_RS07625 (HO345_07620)	1615927..1616076	-	150	WP_253682322.1	hypothetical protein	-
HO345_RS07630 (HO345_07625)	1616104..1616412	+	309	WP_002677634.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HO345_RS07635 (HO345_07630)	1616422..1616730	+	309	WP_253682324.1	NadS family protein	Antitoxin
HO345_RS07640 (HO345_07635)	1616864..1617535	+	672	WP_253682326.1	TetR/AcrR family transcriptional regulator	-
HO345_RS07645 (HO345_07640)	1617624..1618040	+	417	WP_252507806.1	SRPBCC family protein	-
HO345_RS07650 (HO345_07645)	1618164..1618793	-	630	WP_253682328.1	adenylate kinase	-
HO345_RS07655 (HO345_07650)	1618850..1620079	-	1230	WP_253682330.1	cation:dicarboxylase symporter family transporter	-
HO345_RS07660 (HO345_07655)	1620118..1621437	-	1320	WP_253682332.1	leucine-rich repeat domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (43-86)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11736.96 Da
• Isoelectric Point: 10.6864

>T166966 WP_002677634.1 NZ_CP058624:1616104-1616412 [Treponema denticola]
MRIIETPVFTKQINRLLDEDTYKQFKEYLVCNPLKGKLIKGGGGIRKIRWSKKNTGKSGGIRIIYFIKTETKIYLLFAYS
KSDAESITKKQINMLASFIKGL

Nucleotide

• Length: 309 bp

>T166966 NZ_CP074576:2457347-2457841 [Escherichia coli]
ATGATTCAGGCCCCCGAGCCACTCATGGCAAGGCATCAATTCACCTCTTTTTGCTCGGGAGTAGAAACGATGGATAACTG
GCTAAAGCAGAGAGCCCTAAAAAATCAGCTTGCCGGCGCATCACGTACATTTGTTAGCTGTGATACGTATAGCAACGTTT
TGGCATATTATTCGCTTGCTTCCAGCGCCGTTGAAACGTATGTCGCGACAGGACGATTTCGCCGAAATATGCCAGAACCG
ATCCCTGTTGTAGTACTTGGTCGTCTGGCAATAGATAAATCATTACAGGGTCAGGGCATCGATCGTGCGATGGTCAGAGA
TGCCGGTCTACGAGTACTACAGGCGGCTGAAGTCATTGGTATTCGCGGAATGTTGGTCCATGCGTTATCAGACCAGGCCC
GTGAGTTTTATCTGCGAGTGGGGTTTGAACCATCGCCTGTCGATTCTATGATACTGATGGCAACATTGGCTGATTTGCAG
GAATGTCTTAAATAG

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11549.43 Da
• Isoelectric Point: 9.6884

>AT166966 WP_253682324.1 NZ_CP058624:1616422-1616730 [Treponema denticola]
MFKRKKMSDEMFAELLQSVKEAVLIEKGEIPPSRVFEIEPLDIAKIRSKTNKTQEEFASMLNISIGTLRNWEQGRRKPDG
AALSLLKIVSANPQYVESVLQG

Nucleotide

• Length: 309 bp

>AT166966 NZ_CP074576:2457057-2457350 [Escherichia coli]
ATGCCAGCAGCCAATAACATTCCAGCCAGGCGCGAATCTTTAAACTTGCGCATAAAACCCGCAGAACGCGATCTTATTGA
CCGGGCCGCTAAAGCCCGAGGAAAAAATCGCACCGAATTTGTCCTTGAGGCCGCCAGAGCCGCAGCTGAAGAGGCGCTCA
TTGAGCAGAGAATTTTCAGGATCGATTCTGATGATTATCAGCTATTTTTGAATCGTCTGGATCAGCCCCCCGCGCCCAAT
GCTGCATTACGCAAAACCATGCAAACACCCGCGCCGTGGGAGCAAAAAAAATGA

Structures

Toxin

• AlphaFold DB: Q73NN6