Detailed information of TA system TA166959

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 24861..25432
• Replicon: plasmid unnamed1
• Accession: NZ_CP058619
• Organism: Escherichia coli strain JS316

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: HZF20_RS22790
• Protein ID: WP_032323275.1
• Coordinates: 25151..25432 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HZF20_RS22785
• Protein ID: WP_032323276.1
• Coordinates: 24861..25142 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZF20_RS22750	20293..21084	-	792	WP_000532307.1	hypothetical protein	-
HZF20_RS22755	21247..21549	+	303	WP_000162415.1	type II toxin-antitoxin system RelE/ParE family toxin	-
HZF20_RS22760	21620..21958	+	339	WP_000806445.1	HigA family addiction module antidote protein	-
HZF20_RS22765	22015..22374	+	360	WP_032323279.1	transcriptional regulator	-
HZF20_RS22770	22438..23217	+	780	WP_032323278.1	hypothetical protein	-
HZF20_RS22775	23253..24446	-	1194	WP_032323277.1	hypothetical protein	-
HZF20_RS22780	24439..24768	-	330	WP_000542383.1	hypothetical protein	-
HZF20_RS22785	24861..25142	-	282	WP_032323276.1	HigA family addiction module antidote protein	Antitoxin
HZF20_RS22790	25151..25432	-	282	WP_032323275.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HZF20_RS22795	25769..26422	-	654	WP_023908597.1	hypothetical protein	-
HZF20_RS22800	26706..27314	+	609	WP_032323273.1	hypothetical protein	-
HZF20_RS22805	27500..27727	+	228	WP_001396850.1	hypothetical protein	-
HZF20_RS22810	27724..28035	-	312	WP_000717724.1	hypothetical protein	-
HZF20_RS22815	28095..29036	-	942	WP_077697806.1	Rha family transcriptional regulator	-
HZF20_RS22820	29036..29527	-	492	Protein_39	host cell division inhibitor Icd-like protein	-
HZF20_RS22825	29564..29731	-	168	WP_167895685.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..91318	91318

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-86)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10606.14 Da
• Isoelectric Point: 10.2589

>T166959 WP_032323275.1 NZ_CP058619:c25432-25151 [Escherichia coli]
MIKSFRHKGLERFFKTGSTAGIQAKHAVKLRIQLTALNTAKRPDDMSAPGWELHPLKGADLKGHWAISVNGNWRLTFRFE
GEDAILVDYQDYH

Nucleotide

• Length: 282 bp

>T166959 NZ_CP074576:c394927-394550 [Escherichia coli]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGTTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10160.63 Da
• Isoelectric Point: 7.3179

>AT166959 WP_032323276.1 NZ_CP058619:c25142-24861 [Escherichia coli]
MSRMHNPAHPGAVLREYLGDMSVTDAAAALGITRTALSRILNEKAGISADMALRLESALGTSAEMWTGMQSQYELWVASQ
TTRPNIRPLFFHA

Nucleotide

• Length: 282 bp

>AT166959 NZ_CP074576:c395348-394974 [Escherichia coli]
GTGTCAGACACACACCCCGGGACAACGCATCCCGACAACAACAACGACCGCCCCTGGTGGGGGCTCCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGGTATCAGGGGGCAGT
TTAGCGACGCGGATGCGTATCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGCTG
TGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA