Detailed information of TA system TA166958

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 21247..21958
• Replicon: plasmid unnamed1
• Accession: NZ_CP058619
• Organism: Escherichia coli strain JS316

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I2UJZ0
• Locus tag: HZF20_RS22755
• Protein ID: WP_000162415.1
• Coordinates: 21247..21549 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HZF20_RS22760
• Protein ID: WP_000806445.1
• Coordinates: 21620..21958 (+)
• Length: 113 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZF20_RS22725	16266..16946	-	681	WP_191636385.1	hypothetical protein	-
HZF20_RS22730	16930..17835	-	906	WP_089477107.1	recombination-associated protein RdgC	-
HZF20_RS22735	17895..18548	-	654	WP_000828870.1	hypothetical protein	-
HZF20_RS22740	18545..19525	-	981	WP_000046500.1	hypothetical protein	-
HZF20_RS22745	19529..20296	-	768	WP_000203293.1	hypothetical protein	-
HZF20_RS22750	20293..21084	-	792	WP_000532307.1	hypothetical protein	-
HZF20_RS22755	21247..21549	+	303	WP_000162415.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HZF20_RS22760	21620..21958	+	339	WP_000806445.1	HigA family addiction module antidote protein	Antitoxin
HZF20_RS22765	22015..22374	+	360	WP_032323279.1	transcriptional regulator	-
HZF20_RS22770	22438..23217	+	780	WP_032323278.1	hypothetical protein	-
HZF20_RS22775	23253..24446	-	1194	WP_032323277.1	hypothetical protein	-
HZF20_RS22780	24439..24768	-	330	WP_000542383.1	hypothetical protein	-
HZF20_RS22785	24861..25142	-	282	WP_032323276.1	HigA family addiction module antidote protein	-
HZF20_RS22790	25151..25432	-	282	WP_032323275.1	type II toxin-antitoxin system RelE/ParE family toxin	-
HZF20_RS22795	25769..26422	-	654	WP_023908597.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..91318	91318

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (6-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-69)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11818.56 Da
• Isoelectric Point: 9.8739

>T166958 WP_000162415.1 NZ_CP058619:21247-21549 [Escherichia coli]
MTKKINIKDFRDAWLDDFFEFSTPHRKIPPDIHMTLSRKLDIINAATTCKDLRSPPGNRYEELSGKLNGYSSVRVNKQYR
LIFKWVNGKAEDLYLDPHKY

Nucleotide

• Length: 303 bp

>T166958 NZ_CP074576:c323838-323461 [Escherichia coli]
ATGAATACATTACCCGACACTCACGTACGGGAGGCATCGGGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCAACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACAATGA

Antitoxin

Protein

• Length: 113 a.a.
• Molecular weight: 13049.98 Da
• Isoelectric Point: 6.4754

>AT166958 WP_000806445.1 NZ_CP058619:21620-21958 [Escherichia coli]
MKQATRKPTTVGDILLYEYLEPLELKINELAEILHVHRNTVSALVNNNRKLTMDMAYRLAKAFDTSVDFWINLQTAVDLW
EVENDMRVQEELSRINTAEKFISQRNLNKKAA

Nucleotide

• Length: 339 bp

>AT166958 NZ_CP074576:c324262-323885 [Escherichia coli]
GTGTCAGACACACTGCCCGGGACAACGCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCATCACACTGTATGCGAAAGGGCTGACCTGCGAAGCCGATACCCTTGGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCGGCAGCGCCCGCAATCACCGTATAA

Structures

Toxin

• AlphaFold DB: A0A631VBQ3