Detailed information of TA system TA166906

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4987085..4987687
• Replicon: chromosome
• Accession: NZ_CP058596
• Organism: Escherichia coli strain UPEC_U013

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: HYE75_RS24010
• Protein ID: WP_000897302.1
• Coordinates: 4987376..4987687 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HYE75_RS24005
• Protein ID: WP_000356397.1
• Coordinates: 4987085..4987375 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HYE75_RS23980	4983158..4984060	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
HYE75_RS23985	4984057..4984692	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
HYE75_RS23990	4984689..4985618	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
HYE75_RS23995	4985834..4986052	-	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
HYE75_RS24000	4986448..4986726	-	279	WP_001296612.1	hypothetical protein	-
HYE75_RS24005	4987085..4987375	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
HYE75_RS24010	4987376..4987687	-	312	WP_000897302.1	hypothetical protein	Toxin
HYE75_RS24015	4987917..4988825	+	909	WP_001305059.1	alpha/beta hydrolase	-
HYE75_RS24020	4988889..4989830	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
HYE75_RS24025	4989875..4990312	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HYE75_RS24030	4990309..4991181	-	873	WP_000920762.1	virulence factor BrkB family protein	-
HYE75_RS24035	4991175..4991774	-	600	WP_001443191.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T166906 WP_000897302.1 NZ_CP058596:c4987687-4987376 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T166906 NZ_CP074545:77884-78459 [Klebsiella aerogenes]
ATGCGACATTCACCTTATCCGGTGATTTTAGATGCTTGTGTATTATATCCAGCACGGCTCAGGGATTTATTAATGCACTT
AGGGATCGCTGGACTTTATCAACCAAAATGGAGCCGCTTCATTCACGATGAATGGTGCAGGAATCTTCTTATAAATCGGC
CTGATATAGCACCTGAAGCGCTGCGTCGTACCGTTGATCTCATGAATGCAGCTTTACCGGATGCTAATGTAACGGGTTTT
GCAGGATTAATTAACGGACTAGTTTTACCCGACCCTGACGATCGGCATGTCCTTGCTGCGGCAATCAGAGCGAAGGCTGA
AATTATCGTTACGTTAAATCATAAAGACTTTCCAGCAGAATCATTGCTTCCATTTGAAATCGAAACACTTCATCCTGATG
TATTCATATCGGACCTGTTTGACCTTAATCATGCTCTGGCGTTGGAGGCGGTGAGAAGGCAGCGGCAGTCTCTCAAGCAT
CCTCCAATGACCGTCGATGAGTTCCTGGAGATGCTGCTAAAGCAGGGCTTGCCGATGACGGTAAAAGAGTTAGCCAATTA
CCAGTACGCCATATAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT166906 WP_000356397.1 NZ_CP058596:c4987375-4987085 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT166906 NZ_CP074545:77438-77884 [Klebsiella aerogenes]
ATGACTAAACTGAACCTTCCGGACCCGCTGGAGAGTGAACTTGCCCAGCGCGGGCAGCGTGAGCTGGCGGCATACCTTTC
TACTCATCTTGAAACGCAGCGTATTGCGATTGTGGGTGAAGATGACAAAACTCATACCATTGAGTTGCCAACCAGCGCTC
TGACTATGCTGATGGAGATCCTTGGGGAATTGGCTTGTGGCAACGCGGTGCAGATTGTTCCTGTTCATGCTGAACTCACC
ACCCAGGAAGCAGCGAATATACTCAATGTTTCTCGTCCGCATATGGTTAAACTGCTGGAAGCGAGGAAGCTTCCATTTCA
TAAAACTGGACGCCATCGCCGGGTTCTGTTTGCCGATCTGATGGAGTATAAAAAACGTAGGGAACAGGAAAGCCTGGACG
CGATGCAAGCGCTGGCGGATCAGGCGCAGGACTTAGGGATGTATTAA

Structures

Toxin

• AlphaFold DB: S1P416