Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2591156..2591718 Replicon chromosome
Accession NZ_CP058596
Organism Escherichia coli strain UPEC_U013

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag HYE75_RS12145 Protein ID WP_000605675.1
Coordinates 2591156..2591434 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag HYE75_RS12150 Protein ID WP_000781370.1
Coordinates 2591434..2591718 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HYE75_RS12120 2586742..2587173 - 432 WP_000152307.1 peroxiredoxin OsmC -
HYE75_RS12125 2587518..2587733 + 216 WP_000495766.1 biofilm-dependent modulation protein -
HYE75_RS12130 2587835..2587972 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
HYE75_RS12135 2588129..2589826 + 1698 WP_001555531.1 malate dehydrogenase -
HYE75_RS12140 2589960..2590970 + 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
HYE75_RS12145 2591156..2591434 + 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HYE75_RS12150 2591434..2591718 + 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
HYE75_RS12155 2591947..2592600 - 654 WP_000045646.1 formate dehydrogenase-N subunit gamma -
HYE75_RS12160 2592593..2593477 - 885 WP_001240584.1 formate dehydrogenase N subunit beta -
HYE75_RS12165 2593490..2596537 - 3048 WP_011076399.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T166892 WP_000605675.1 NZ_CP058596:2591156-2591434 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T166892 NZ_CP074540:c6116-5811 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCTGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT166892 WP_000781370.1 NZ_CP058596:2591434-2591718 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT166892 NZ_CP074540:c6336-6118 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGTGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References