Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1265940..1266667 Replicon chromosome
Accession NZ_CP058596
Organism Escherichia coli strain UPEC_U013

Toxin (Protein)


Gene name higB Uniprot ID V0YLE2
Locus tag HYE75_RS06070 Protein ID WP_000547555.1
Coordinates 1265940..1266251 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0H2V9Z2
Locus tag HYE75_RS06075 Protein ID WP_000126293.1
Coordinates 1266248..1266667 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HYE75_RS06040 1261081..1262790 + 1710 WP_001288134.1 formate hydrogenlyase subunit HycE -
HYE75_RS06045 1262800..1263342 + 543 WP_000493797.1 formate hydrogenlyase subunit HycF -
HYE75_RS06050 1263342..1264109 + 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
HYE75_RS06055 1264106..1264516 + 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
HYE75_RS06060 1264509..1264979 + 471 WP_000132956.1 hydrogenase maturation peptidase HycI -
HYE75_RS06065 1265022..1265777 + 756 WP_001305268.1 hypothetical protein -
HYE75_RS06070 1265940..1266251 + 312 WP_000547555.1 type II toxin-antitoxin system HigB family toxin Toxin
HYE75_RS06075 1266248..1266667 + 420 WP_000126293.1 helix-turn-helix domain-containing protein Antitoxin
HYE75_RS06080 1266759..1267187 - 429 WP_000536064.1 hypothetical protein -
HYE75_RS06085 1267572..1268099 + 528 WP_001078777.1 electron transport protein HydN -
HYE75_RS06090 1268252..1270504 + 2253 WP_001107659.1 carbamoyltransferase HypF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12439.18 Da        Isoelectric Point: 9.5146

>T166880 WP_000547555.1 NZ_CP058596:1265940-1266251 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

>T166880 NZ_CP058596:1265940-1266251 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTGTGCGCGCAAATATCCTAACGACGCTCTGGCTCTACACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAAATGCGAACGGCTTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATCACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15426.46 Da        Isoelectric Point: 4.4596

>AT166880 WP_000126293.1 NZ_CP058596:1266248-1266667 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTIPHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT166880 NZ_CP058596:1266248-1266667 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGTGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCAGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGCCAGATCTTGTCTGGACAACGATCCTTGACGATACCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0YLE2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H2V9Z2

References