Detailed information of TA system TA166865

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 72002..72428
• Replicon: plasmid pEC1-1
• Accession: NZ_CP058575
• Organism: Escherichia coli strain EC1

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: HYH05_RS24935
• Protein ID: WP_001312861.1
• Coordinates: 72270..72428 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	72002..72226 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HYH05_RS24890	68275..68840	+	566	Protein_81	class I SAM-dependent methyltransferase	-
HYH05_RS24895	68866..69078	+	213	WP_001348622.1	hypothetical protein	-
HYH05_RS24900	69488..69694	+	207	WP_000275856.1	hypothetical protein	-
HYH05_RS24905	69720..70259	+	540	WP_000290840.1	single-stranded DNA-binding protein	-
HYH05_RS24910	70327..70560	+	234	WP_000005990.1	DUF905 family protein	-
HYH05_RS24915	70588..70785	+	198	Protein_86	hypothetical protein	-
HYH05_RS24920	70840..71274	+	435	WP_000845937.1	conjugation system SOS inhibitor PsiB	-
HYH05_RS24925	71271..71990	+	720	WP_001276238.1	plasmid SOS inhibition protein A	-
HYH05_RS24930	72002..72190	-	189	WP_001299721.1	hypothetical protein	-
-	72002..72226	+	225	NuclAT_0	-	Antitoxin
-	72002..72226	+	225	NuclAT_0	-	Antitoxin
-	72002..72226	+	225	NuclAT_0	-	Antitoxin
-	72002..72226	+	225	NuclAT_0	-	Antitoxin
HYH05_RS24935	72270..72428	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
HYH05_RS24940	73118..73324	+	207	WP_000275857.1	hypothetical protein	-
HYH05_RS24945	73349..73636	+	288	WP_000107535.1	hypothetical protein	-
HYH05_RS24950	73753..74574	+	822	WP_001234426.1	DUF945 domain-containing protein	-
HYH05_RS24955	74871..75473	-	603	WP_013362798.1	transglycosylase SLT domain-containing protein	-
HYH05_RS24960	75794..76177	+	384	WP_001151524.1	relaxosome protein TraM	-
HYH05_RS24965	76364..77053	+	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / aac(3)-IId / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / mph(A)	-	1..122379	122379

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T166865 WP_001312861.1 NZ_CP058575:72270-72428 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T166865 NZ_CP074530:c7691-7386 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTACGCCTATAAAAGAGAAAGCCGCTACCGTCTGTTCGTCGACGTGCAGAGTGACATTATTGACAC
CCCGGGACGCCGGATGGTGATCCCGCTGGTCAGCGCCCGCCTGCTGTCCGACAAAGTCTCGCGTGAGCTTTATCCGGTGG
TGTCAGTCGGAGGTGACCCTTATCGTCTGATGACCACGGATATGGCCAGTGTGCCGGCCACTGTCATCGGGGAAGAAGTG
GCCGATCTCAGCCACCTGGAGAATGACATCCAGAACGCCATCAACCTGATGTTCCGGGGGATCTGA

Antitoxin

Nucleotide

• Length: 225 bp

>AT166865 NZ_CP058575:72002-72226 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin