Detailed information of TA system TA166858

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symE-agrB/SymE(toxin)
• Location: 4307340..4307752
• Replicon: chromosome
• Accession: NZ_CP058574
• Organism: Escherichia coli strain EC1

Toxin (Protein)

• Gene name: symE
• Uniprot ID: U9YSY7
• Locus tag: HYH05_RS20820
• Protein ID: WP_000132601.1
• Coordinates: 4307411..4307752 (+)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	agrB
Locus tag	-
Coordinates	4307340..4307416 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HYH05_RS20810	4304203..4305792	+	1590	WP_001063200.1	type I restriction-modification system methyltransferase	-
HYH05_RS20815	4305789..4307183	+	1395	WP_001272445.1	type I restriction-modification system specificity subunit	-
-	4307340..4307416	-	77	NuclAT_12	-	Antitoxin
-	4307340..4307416	-	77	NuclAT_12	-	Antitoxin
-	4307340..4307416	-	77	NuclAT_12	-	Antitoxin
-	4307340..4307416	-	77	NuclAT_12	-	Antitoxin
-	4307340..4307416	-	77	NuclAT_13	-	Antitoxin
-	4307340..4307416	-	77	NuclAT_13	-	Antitoxin
-	4307340..4307416	-	77	NuclAT_13	-	Antitoxin
-	4307340..4307416	-	77	NuclAT_13	-	Antitoxin
HYH05_RS20820	4307411..4307752	+	342	WP_000132601.1	endoribonuclease SymE	Toxin
HYH05_RS20825	4307914..4309293	+	1380	WP_000443951.1	5-methylcytosine-specific restriction endonuclease subunit McrB	-
HYH05_RS20830	4309293..4310339	+	1047	WP_000437621.1	5-methylcytosine-specific restriction endonuclease system specificity protein McrC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12203.02 Da
• Isoelectric Point: 8.5012

>T166858 WP_000132601.1 NZ_CP058574:4307411-4307752 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRHVTVGYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAEESE
LMQSLRQVCKLSARKQKQVQAFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T166858 NZ_CP074528:4153447-4153665 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Nucleotide

• Length: 77 bp

>AT166858 NZ_CP058574:c4307416-4307340 [Escherichia coli]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: U9YSY7

Antitoxin