Detailed information of TA system TA166829

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 13073..13337
• Replicon: plasmid pEC2-1
• Accession: NZ_CP058572
• Organism: Escherichia coli strain EC2

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Y6M3
• Locus tag: HX136_RS25105
• Protein ID: WP_001331364.1
• Coordinates: 13073..13225 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	13280..13337 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HX136_RS25080	8350..10518	+	2169	WP_021537350.1	DotA/TraY family protein	-
HX136_RS25085	10592..11242	+	651	WP_001178506.1	plasmid IncI1-type surface exclusion protein ExcA	-
HX136_RS25090	11314..11523	-	210	WP_000062603.1	HEAT repeat domain-containing protein	-
HX136_RS25095	11915..12091	+	177	WP_001054904.1	hypothetical protein	-
HX136_RS25100	12750..13001	+	252	WP_001291964.1	hypothetical protein	-
HX136_RS25105	13073..13225	-	153	WP_001331364.1	Hok/Gef family protein	Toxin
-	13280..13337	+	58	NuclAT_0	-	Antitoxin
-	13280..13337	+	58	NuclAT_0	-	Antitoxin
-	13280..13337	+	58	NuclAT_0	-	Antitoxin
-	13280..13337	+	58	NuclAT_0	-	Antitoxin
HX136_RS25110	13517..14725	+	1209	WP_000121273.1	IncI1-type conjugal transfer protein TrbA	-
HX136_RS25115	14744..15814	+	1071	WP_000151583.1	IncI1-type conjugal transfer protein TrbB	-
HX136_RS25120	15807..18098	+	2292	WP_001289276.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	ant(3'')-Ia / sul3 / fosA3 / blaTEM-1B	-	1..109494	109494

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5775.13 Da
• Isoelectric Point: 8.7948

>T166829 WP_001331364.1 NZ_CP058572:c13225-13073 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T166829 NZ_CP074519:1850598-1850700 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Nucleotide

• Length: 58 bp

>AT166829 NZ_CP058572:13280-13337 [Escherichia coli]
GCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A5W9M382

Antitoxin