Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 4094081..4094775 Replicon chromosome
Accession NZ_CP058571
Organism Escherichia coli strain EC2

Toxin (Protein)


Gene name yafO Uniprot ID A0A3P5GWS5
Locus tag HX136_RS20150 Protein ID WP_001263488.1
Coordinates 4094081..4094479 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag HX136_RS20155 Protein ID WP_000554758.1
Coordinates 4094482..4094775 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- 4089669..4089749 - 81 NuclAT_13 - -
- 4089669..4089749 - 81 NuclAT_13 - -
- 4089669..4089749 - 81 NuclAT_13 - -
- 4089669..4089749 - 81 NuclAT_13 - -
HX136_RS20125 4090345..4090803 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
HX136_RS20130 4091064..4092521 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
HX136_RS20135 4092578..4093099 - 522 Protein_3948 peptide chain release factor H -
HX136_RS20140 4093095..4093301 - 207 Protein_3949 RtcB family protein -
HX136_RS20145 4093619..4094071 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
HX136_RS20150 4094081..4094479 - 399 WP_001263488.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
HX136_RS20155 4094482..4094775 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
HX136_RS20160 4094827..4095882 - 1056 WP_001226164.1 DNA polymerase IV -
HX136_RS20165 4095953..4096738 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
HX136_RS20170 4096683..4098422 + 1740 WP_001334802.1 flagellar biosynthesis protein FlhA -
HX136_RS20175 4098646..4099143 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15559.90 Da        Isoelectric Point: 6.9798

>T166826 WP_001263488.1 NZ_CP058571:c4094479-4094081 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQEAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T166826 NZ_CP074519:331368-331736 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGTATTACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT166826 WP_000554758.1 NZ_CP058571:c4094775-4094482 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT166826 NZ_CP074519:331150-331371 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCTGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3P5GWS5

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References