Detailed information of TA system TA166809

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 1926002..1926504
• Replicon: chromosome
• Accession: NZ_CP058571
• Organism: Escherichia coli strain EC2

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: U9YDH0
• Locus tag: HX136_RS09295
• Protein ID: WP_000767829.1
• Coordinates: 1926250..1926504 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: S1Q063
• Locus tag: HX136_RS09290
• Protein ID: WP_001259255.1
• Coordinates: 1926002..1926253 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HX136_RS09265	1921547..1922608	-	1062	WP_001019193.1	histidinol-phosphate transaminase	-
HX136_RS09270	1922605..1923909	-	1305	WP_000009605.1	histidinol dehydrogenase	-
HX136_RS09275	1923915..1924814	-	900	WP_000886595.1	ATP phosphoribosyltransferase	-
HX136_RS09280	1924971..1925429	-	459	WP_000426724.1	IS200/IS605 family transposase	-
HX136_RS09285	1925669..1925719	-	51	WP_001364200.1	his operon leader peptide	-
HX136_RS09290	1926002..1926253	+	252	WP_001259255.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
HX136_RS09295	1926250..1926504	+	255	WP_000767829.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
HX136_RS09300	1926587..1927411	+	825	WP_000754737.1	SDR family oxidoreductase	-
HX136_RS09305	1927457..1928386	+	930	WP_032239902.1	LysR family transcriptional regulator	-
HX136_RS09310	1928601..1928663	+	63	WP_010723108.1	membrane protein YoeI	-
HX136_RS09315	1928653..1930011	+	1359	WP_032270653.1	putrescine/proton symporter PlaP	-
HX136_RS09320	1930190..1931248	+	1059	WP_032270652.1	transport protein YeeE	-
HX136_RS09325	1931262..1931489	+	228	WP_000985273.1	sulfurtransferase TusA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1924971..1925429	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10183.58 Da
• Isoelectric Point: 8.0353

>T166809 WP_000767829.1 NZ_CP058571:1926250-1926504 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T166809 NZ_CP074448:887110-887463 [Enterobacter asburiae]
GTGTGGGCAATTAACACGACGGACAGGTTTGATCGCTGGTTCACTTCGCTTGACGATACCGATCGCGCCAGCGTACTTGC
AGCACTGCTTGTTTTGCGAGAGAAGGGGCCGGGTTTATCGAGACCCTACGCTGATACGCTTAAAGGCTCTCGTTACAGCA
ACATGAAAGAGCTACGTATTCAGAGTAAGGGCGATCCCTTGCGGGCGTTTTTCGCCTTTGATCCAAACCGCACGGGTATC
GTGCTTTGTGCGGGCAATAAGGTCGGCAATGAAAGGCGATTCTACGATGAGATGCTTCTCGTTGCTGACCGGGAATTCAC
TCACTGGCTGGATAGATTAAAAGAGAGGGAATAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9307.58 Da
• Isoelectric Point: 4.7954

>AT166809 WP_001259255.1 NZ_CP058571:1926002-1926253 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT166809 NZ_CP074448:887468-887770 [Enterobacter asburiae]
ATGGGAAGAACCCTTGAGCAGCTTATAGCGGATGAAAAGCCGGAGGTTGTCGCCAGCGCTCAGGCGATAGCAACGGATAT
TTTGCTCAACATTCATCTTGCTGAGTTACGCGAGAAAGTACAAAAAACGCAGGTAGAAATGGCGCAGGCGTTGGGTATCA
GGCAACCTACCGTTGCGGTAATGGAAAAGCCCGGGCGCGATTTGAAACTCTCTTCTCTCAAGCGCTACGTTGAGGCTGCG
GGCGGAAAGCTGCGGCTTGATGTGGAGCTGCCGGATGGCTCGCACTATGAGTTTGTGCTTTAG

Structures

Toxin

• PDB: 6NY6
• PDB: 2A6S
• PDB: 6OXA
• PDB: 2A6R
• PDB: 4V8X
• PDB: 6OTR
• PDB: 6OXI
• PDB: 2A6Q

Antitoxin

• AlphaFold DB: A0A7U9LNR0