Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 659224..659951 Replicon chromosome
Accession NZ_CP058571
Organism Escherichia coli strain EC2

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag HX136_RS03120 Protein ID WP_179231284.1
Coordinates 659637..659951 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag HX136_RS03115 Protein ID WP_000560266.1
Coordinates 659224..659640 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HX136_RS03105 654384..656735 + 2352 WP_000695487.1 alpha-glucosidase -
HX136_RS03110 657161..659179 + 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
HX136_RS03115 659224..659640 - 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
HX136_RS03120 659637..659951 - 315 WP_179231284.1 type II toxin-antitoxin system toxin HigB Toxin
HX136_RS03125 660235..661371 - 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
HX136_RS03130 661456..661959 + 504 WP_001333820.1 M48 family metallopeptidase -
HX136_RS03135 662036..662728 + 693 WP_000942548.1 vancomycin high temperature exclusion protein -
HX136_RS03140 662807..663793 + 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12050.05 Da        Isoelectric Point: 9.7553

>T166805 WP_179231284.1 NZ_CP058571:c659951-659637 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHCTKGKK

Download         Length: 315 bp

>T166805 NZ_CP074446:c4521691-4521158 [Klebsiella aerogenes]
ATGGATCAGCTACTGACGATTGAGATGATTGCCGATGAGTTCCGCTACGACATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAACATCTCAAGCGACAGCACGATGGACAGATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGCTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGCGGCATGCTGCCGTCTAAAACTCAG
CAAAAGAAAATTCCGTATCAAAATGCGCCAAGCGTAACTCTGGGGCGGCTGGCCATTGATAAATCCGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTCGCCCATGCGATGCGAGTGGTGTGGGGGGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCTCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGATGATGAGTCATAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT166805 WP_000560266.1 NZ_CP058571:c659640-659224 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT166805 NZ_CP074446:c4521968-4521702 [Klebsiella aerogenes]
ATGCCCGCACTCAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACTCAACCGCCAGCACCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References