Detailed information of TA system TA166793

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 37611..38338
• Replicon: plasmid pS165-1.1
• Accession: NZ_CP058549
• Organism: Klebsiella pneumoniae strain S165-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: HXT63_RS26580
• Protein ID: WP_011251285.1
• Coordinates: 37611..37922 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HXT63_RS26585
• Protein ID: WP_011251286.1
• Coordinates: 37919..38338 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HXT63_RS26550	33039..33419	-	381	Protein_39	integrase core domain-containing protein	-
HXT63_RS26555	33476..34434	-	959	Protein_40	IS5-like element IS903B family transposase	-
HXT63_RS26560	34467..35276	-	810	Protein_41	IS481-like element ISKpn28 family transposase	-
HXT63_RS26565	35349..35510	+	162	WP_004118124.1	type I toxin-antitoxin system Hok family toxin	-
HXT63_RS26570	35746..36714	-	969	WP_237086706.1	IS5 family transposase	-
HXT63_RS26575	36969..37406	+	438	Protein_44	DDE-type integrase/transposase/recombinase	-
HXT63_RS26580	37611..37922	+	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
HXT63_RS26585	37919..38338	+	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
HXT63_RS26590	38485..39452	+	968	Protein_47	IS5 family transposase	-
HXT63_RS26595	39497..40147	-	651	WP_237086707.1	hypothetical protein	-
HXT63_RS26600	40257..40787	-	531	WP_228292664.1	hypothetical protein	-
HXT63_RS26605	41206..41775	+	570	WP_228292663.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul2 / floR / dfrA12 / aadA2 / aph(3')-Ia / tet(A)	-	1..169990	169990
	-	inside	IScluster/Tn	-	-	18225..45115	26890

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T166793 WP_011251285.1 NZ_CP058549:37611-37922 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T166793 NZ_CP074442:c2863565-2862243 [Klebsiella pneumoniae]
ATGGCGACGCTTACCACCTGGATGAACAATGTGCGCGTGGGAACCTTAACCCGCCAGGCTAACGGGGCGCACAGTTTTCG
CTATGACGAAGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCCCTCTCACTGCCGCTGCAGTATGGCAATATCACTG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTGCCCGACAGCCCGCAGGTCCGCGACCGGATCGTCAGGCGCTATCAG
GCGCGCTCGAAGCAGCCGTTCGATCTGCTGGCCGAAGTCGGGCGGGATAGCGTAGGGGCGGTGACCCTCCTGCCGCCGGG
GGAAGAGGCGCACCTGGAAGGATTACGCTGGCAGACGCTGGACGAGGCGCAGCTCACCGCTTTGCTGACCGCTTACCAGT
CGGATATCCCCCTCGGGATGATTACCAGCCAGGACGATTTCCGTATCTCGGTGGCCGGCGCCCAGGAGAAAACGGCGCTG
CTGCGGATGGGCGAGCAGTGGTGTATTCCGCAGGGCGCCACGCCGACCACGCATATCATTAAGCTGCCCATCGGCGAGAT
CAAGCAGCCCAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAATGAGTATCTGTGCCTGGCGCTGGCCAGAGAACTGG
GCCTGGCGGTGCCGGAAGCGGAGATCATCACCACCCCGCGGATCCGCGCCCTGGCGGTAACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGACGGGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCTTTTGGTCTTCCTTCAGCGATGAAATA
TGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCGAGTGAAGCGCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTCTTCCAGTGGCTGACGGGGGCGACGGACGGTCACGCAAAAAACTTCTCAATTTACCTGTTG
CCCGGCGGCAGCTACCGCTTAACACCGTTCTACGACATTATTTCGGCGTTTCCGGTGCTGGGCGGCACGGGACTGCATCT
GCGCGATCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGTAAAACAGAGATTAACGCCATCTATCCGCGTCATT
TTCTGGCGACGGCCAAAGCGGTGAACTTTCCCCGGGAGCAGATGCTGGCGATCCTCCAGGAGTTTGCCGGGCATGTGCCG
CAGGCAATCGAAAGCGCTCGCCGGACGCTGCCGGCGGACTTTTCCAGCCACGTCTGGCAGGCCATTACAGAGAACATGCT
GAAGCTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGCTATAG

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT166793 WP_011251286.1 NZ_CP058549:37919-38338 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT166793 NZ_CP074442:c2863831-2863565 [Klebsiella pneumoniae]
ATGAATTCCCCGCAGATATACAGTCCCGTGCAGCTGGCCAATTACCTGAAGCTGCTGCGCCAGCAGAACCACTGGACCCA
GGATGCGCTGGCCAGGCGGATCGGCATTAAGCAGGCCACGCTGTCCAATTTTGAAAATCACCCCGACAAAACCACCTTAA
CCACGCTGTTTAAGATCCTCCAGTCGCTGGGGGTGGTGATGACGCTGAACGCCAAAGCTGCTGAGACGGCGCCGGCTCCC
GATAACAACACGGACCTTGACTGGTAA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1