Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3492795..3493392 Replicon chromosome
Accession NZ_CP058548
Organism Klebsiella pneumoniae strain S165-1

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag HXT63_RS17070 Protein ID WP_004142563.1
Coordinates 3492795..3493112 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag HXT63_RS17075 Protein ID WP_004142561.1
Coordinates 3493105..3493392 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HXT63_RS17055 (3488495) 3488495..3489922 - 1428 WP_009308097.1 MFS transporter -
HXT63_RS17060 (3490031) 3490031..3490897 + 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
HXT63_RS17065 (3491567) 3491567..3492610 + 1044 WP_040153598.1 DUF2157 domain-containing protein -
HXT63_RS17070 (3492795) 3492795..3493112 + 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HXT63_RS17075 (3493105) 3493105..3493392 + 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
HXT63_RS17080 (3493657) 3493657..3494295 - 639 WP_064277739.1 oxygen-insensitive NAD(P)H nitroreductase -
HXT63_RS17085 (3494415) 3494415..3494783 - 369 WP_040171587.1 MmcQ/YjbR family DNA-binding protein -
HXT63_RS17090 (3494780) 3494780..3495364 - 585 WP_237086602.1 TetR/AcrR family transcriptional regulator -
HXT63_RS17095 (3495544) 3495544..3496659 + 1116 WP_012737592.1 MBL fold metallo-hydrolase -
HXT63_RS17100 (3496690) 3496690..3497031 + 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
HXT63_RS17105 (3497049) 3497049..3497297 - 249 WP_002893037.1 DUF1158 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T166790 WP_004142563.1 NZ_CP058548:3492795-3493112 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T166790 NZ_CP074442:873337-873747 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT166790 WP_004142561.1 NZ_CP058548:3493105-3493392 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT166790 NZ_CP074442:873090-873356 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References